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验证数据展示

  • WB analysis of mouse testis using 16077-1-AP

    WB analysis of mouse testis using 16077-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16077-1-AP (ACSBG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 16077-1-AP

    IHC staining of mouse testis using 16077-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16077-1-AP (ACSBG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 16077-1-AP

    IHC staining of mouse testis using 16077-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16077-1-AP (ACSBG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ACSBG1 Polyclonal antibody

ACSBG1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16077-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ACSBG1, BG1, BGM, GR LACS, hBG1, hsBG, hsBGM, KIAA0631, Lipodisin, LPD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16077-1-AP targets ACSBG1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 ACSBG1 fusion protein Ag9043 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acyl-CoA synthetase bubblegum family member 1
别名 ACSBG1, BG1, BGM, GR LACS, hBG1, hsBG, hsBGM, KIAA0631, Lipodisin, LPD
计算分子量 724 aa, 81 kDa
观测分子量 81 kDa
GenBank蛋白编号BC009289
基因名称 ACSBG1
Gene ID (NCBI) 23205
RRIDAB_2878214
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96GR2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ACSBG1 antibody 16077-1-APDownload protocol
IHC protocol for ACSBG1 antibody 16077-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Endocrinology

mTOR regulates mineralocorticoid receptor transcriptional activity by ULK1- dependent and independent mechanisms

Authors - Yusuf Ali
View Article