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验证数据展示

  • WB analysis of L02 using 17316-1-AP (same clone as 17316-1-PBS)

    WB analysis of L02 using 17316-1-AP (same clone as 17316-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 17316-1-AP (ACOT4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17316-1-PBS in a different storage buffer formulation.

  • WB analysis using 17316-1-AP (same clone as 17316-1-PBS)

    WB analysis using 17316-1-AP (same clone as 17316-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17316-1-AP (ACOT4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17316-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 17316-1-AP (same clone as 17316-1-PBS)

    IHC staining of mouse kidney using 17316-1-AP (same clone as 17316-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17316-1-AP (ACOT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17316-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 17316-1-AP (same clone as 17316-1-PBS)

    IHC staining of mouse kidney using 17316-1-AP (same clone as 17316-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17316-1-AP (ACOT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17316-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 17316-1-AP (same clone as 17316-1-PBS)

    IF Staining of A549 using 17316-1-AP (same clone as 17316-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ACOT4 antibody (17316-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17316-1-PBS in a different storage buffer formulation.

ACOT4 Polyclonal antibody, PBS Only

ACOT4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 17316-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

17316-1-PBS targets ACOT4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag11286

Product name: Recombinant human ACOT4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2-225 aa of BC031799

Sequence: NNMDNISLEYFEEAVCYMLQHPQVKGPGIGLLGISLGADICLSMASFLKNVSATVSINGSGISGNTAINYKHSSIPPLGYDLRRIKVAFSGLVDIVDIRNALVGGYKNPSMIPIEKAQGPILLIVGQDDHNWRSELYAQTVSERLQAHGKEKPQIICYPGTGHYIEPPYFPLCPASLHRLLNKHVIWGGEPRAHSKAQEDAWKQILAFFCKHLGGTQKTAVPKL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acyl-CoA thioesterase 4
别名
计算分子量 421 aa, 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC031799
基因名称 ACOT4
Gene ID (NCBI) 122970
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N9L9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACOT4, also known as PET2B and PTEIB, is an acyl-CoA thioesterases which catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), regulating their respective intracellular levels. ACOT4 has the strongest expression in liver and kidney and weaker expression in placenta, heart, and muscle(PMID: 16940157).

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