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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of L02 using 17316-1-AP (same clone as 17316-1-PBS)

    WB analysis of L02 using 17316-1-AP (same clone as 17316-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 17316-1-AP (ACOT4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17316-1-PBS in a different storage buffer formulation.

  • WB analysis using 17316-1-AP (same clone as 17316-1-PBS)

    WB analysis using 17316-1-AP (same clone as 17316-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17316-1-AP (ACOT4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17316-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 17316-1-AP (same clone as 17316-1-PBS)

    IHC staining of mouse kidney using 17316-1-AP (same clone as 17316-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17316-1-AP (ACOT4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17316-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 17316-1-AP (same clone as 17316-1-PBS)

    IHC staining of mouse kidney using 17316-1-AP (same clone as 17316-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17316-1-AP (ACOT4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17316-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 17316-1-AP (same clone as 17316-1-PBS)

    IF Staining of A549 using 17316-1-AP (same clone as 17316-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ACOT4 antibody (17316-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17316-1-PBS in a different storage buffer formulation.

ACOT4 Polyclonal antibody, PBS Only

ACOT4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 17316-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

17316-1-PBS targets ACOT4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 ACOT4 fusion protein Ag11286 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acyl-CoA thioesterase 4
别名
计算分子量 421 aa, 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC031799
基因名称 ACOT4
Gene ID (NCBI) 122970
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N9L9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACOT4, also known as PET2B and PTEIB, is an acyl-CoA thioesterases which catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), regulating their respective intracellular levels. ACOT4 has the strongest expression in liver and kidney and weaker expression in placenta, heart, and muscle(PMID: 16940157).