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验证数据展示

  • WB analysis of A549 using 14304-1-AP (same clone as 14304-1-PBS)

    WB analysis of A549 using 14304-1-AP (same clone as 14304-1-PBS)

    WB result of ACK1 antibody (14304-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACK1 transfected A549 cells. This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

  • WB analysis of SH-SY5Y using 14304-1-AP (same clone as 14304-1-PBS)

    WB analysis of SH-SY5Y using 14304-1-AP (same clone as 14304-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 14304-1-AP (same clone as 14304-1-PBS)

    IHC staining of mouse kidney using 14304-1-AP (same clone as 14304-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14304-1-AP (ACK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TNK2/ACK1 antibody included
  • IHC staining of mouse kidney using 14304-1-AP (same clone as 14304-1-PBS)

    IHC staining of mouse kidney using 14304-1-AP (same clone as 14304-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14304-1-AP (ACK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14304-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TNK2/ACK1 antibody included
  • Featured Product
  • KD/KO验证

ACK1 Polyclonal antibody, PBS Only

ACK1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14304-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

TNK2, ACK, ACK 1, ACK-1, Activated CDC42 kinase 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TNK2/ACK1 Ready-To-Use IHC Kit. TNK2/ACK1 primary antibody included.

产品信息

14304-1-PBS targets ACK1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5419

Product name: Recombinant human ACK1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-385 aa of BC028164

Sequence: MQPEEGTGWLLELLSEVQLQQYFLRLRDDLNVTRLSHFEYVKNEDLEKIGMGRPGQRRLWEAVKRRKALCKRKSWMSKVFSGKRLEAEFPPHHSQSTFRKTSPAPGGPAGEGPLQSLTCLIGEKDLRLLEKLGDGSFGVVRRGEWDAPSGKTVSVAVKCLKPDVLSQPEAMDDFIREVNAMHSLDHRNLIRLYGVVLTPPMKMVTELAPLGSLLDRLRKHQGHFLLGTLSRYAVQVAEGMGYLESKRFIHRDLAARNLLLATRDLVKIGDFGLMRALPQNDDHYVMQEHRKVPFAWCAPESLKTRTFSHASDTWMFGVTLWEMFTYGQEPWIGLNGSQILHKIDKEGERLPRPEDCPQDIYNVMVQCWAHKPEDRPTFVALRDFL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tyrosine kinase, non-receptor, 2
别名 TNK2, ACK, ACK 1, ACK-1, Activated CDC42 kinase 1
计算分子量 115 kDa
观测分子量 70 kDa
GenBank蛋白编号BC028164
基因名称 TNK2
Gene ID (NCBI) 10188
RRIDAB_11041713
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ07912
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TNK2, also named ACK1, belongs to the protein kinase superfamily and Tyr protein kinase family. It is a non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, and cell proliferation. TNK2 transduces extracellular signals to cytosolic and nuclear effectors. It is a downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. TNK2 confers metastatic properties on cancer cells by negatively regulating tumor suppressors such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. The 60-70kd band is the isoform 2 of TNK2. Isoform 1 and 3 is about 120-125kd.