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CoraLite® Plus 488-conjugated ACF1 Monoclonal antibody

ACF1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C4F8

Cat No : CL488-67947

Print datasheet

Synonyms

ACF1, BAZ1A, CHRAC subunit ACF1, DKFZp586E0518, FLJ14383, hACF1, hWALp1, WALp1, WCRF180

验证数据展示

  • FC experiment of HeLa using CL488-67947

    FC experiment of HeLa using CL488-67947

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-HUMAN ACF1 (CL488-67947, Clone:1C4F8) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67947-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67947 targets ACF1 in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra)
Tested Reactivity Human
Immunogen ACF1 fusion protein Ag30607 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name bromodomain adjacent to zinc finger domain, 1A
Synonyms ACF1, BAZ1A, CHRAC subunit ACF1, DKFZp586E0518, FLJ14383, hACF1, hWALp1, WALp1, WCRF180
Calculated Molecular Weight 179 kDa
GenBank Accession NumberNM_013448
Gene Symbol BAZ1A
Gene ID (NCBI) 11177
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NRL2
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 ACF1 antibody CL488-67947Download protocol
Standard Protocols
Click here to view our Standard Protocols