验证数据展示
经过测试的应用
Positive WB detected in | Recombinant Protein |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
60001-1-Ig targets 6*his,His tag in WB, ELISA applications and shows reactivity with recombinant protein samples.
经测试应用 | WB, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | recombinant protein |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Mouse / IgG2b |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | 6*His, His Tag |
别名 | |
计算分子量 | 1 kDa |
基因名称 | His Tag |
Gene ID (NCBI) | 5 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | N/A |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tags are most often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. As is the case with other protein tag systems, this polyhistidine tag can often be cleaved at sites recognized by proteases such as thrombin and enterokinases to isolate the protein of interest. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.
发表文章
Species | Application | Title |
---|---|---|
J Exp Bot Overexpression of the aspartic protease ASPG1 gene confers drought avoidance in Arabidopsis. | ||
Oncol Rep Sperm‑associated antigen 4 (SPAG4) as a new cancer marker interacts with Nesprin3 to regulate cell migration in lung carcinoma. | ||
J Bacteriol No fitness cost entailed by type VI secretion system synthesis, assembly, contraction, or disassembly in enteroaggregative Escherichia coli | ||
J Biol Chem A CASPR1-ATP1B3 protein interaction modulates plasma membrane localization of Na+/K+-ATPase in brain microvascular endothelial cells. |