6*His, His-tag Monoclonal antibody

6*his,His tag Monoclonal Antibody for WB, ELISA
Cat No. 60001-1-Ig

产品说明书

宿主/亚型

Mouse / IgG2b

种属反应性

recombinant protein

应用

WB, ELISA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inRecombinant Protein

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

60001-1-Ig targets 6*his,His tag in WB, ELISA applications and shows reactivity with recombinant protein samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 recombinant protein
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 6*His, His Tag
别名
计算分子量 1 kDa
基因名称 His Tag
Gene ID (NCBI) 5
偶联类型 Unconjugated
形式 Liquid
纯化方式N/A
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tags are most often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. As is the case with other protein tag systems, this polyhistidine tag can often be cleaved at sites recognized by proteases such as thrombin and enterokinases to isolate the protein of interest. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.

发表文章

SpeciesApplicationTitle

Nat Commun

Caspr1 is a host receptor for meningitis-causing Escherichia coli.

Authors - Wei-Dong Zhao
WB

J Exp Bot

Overexpression of the aspartic protease ASPG1 gene confers drought avoidance in Arabidopsis.

Authors - Yao Xuan X
WB

Oncol Rep

Sperm‑associated antigen 4 (SPAG4) as a new cancer marker interacts with Nesprin3 to regulate cell migration in lung carcinoma.

Authors - Ying Ji

J Bacteriol

No fitness cost entailed by type VI secretion system synthesis, assembly, contraction, or disassembly in enteroaggregative Escherichia coli

Authors - Boris Taillefer

J Biol Chem

A CASPR1-ATP1B3 protein interaction modulates plasma membrane localization of Na+/K+-ATPase in brain microvascular endothelial cells.

Authors - Shu-Hong Zhang
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