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(-)-Blebbistatin

化合物(-)-Blebbistatin

CAS号

856925-71-8

分子式

C18H16N2O2

主要靶点

Myosin|ATPase

仅限科研使用

Cat No : CM05239

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Synonyms

(S)-(-)-Blebbistatin

验证数据展示

  • CAS No.: 856925-71-8

    (-)-Blebbistatin
    CAS#: 856925-71-8

产品信息

(-)-Blebbistatin is an S enantiomer of blebbistatin. It is a potent and selective myosin II inhibitor with IC50 ranging from 0.5 to 5 μM.

CAS号 856925-71-8
分子式 C18H16N2O2
主要靶点 Myosin|ATPase
主要通路 细胞骨架|离子通道
分子量 292.33
纯度 99.59%， 此纯度可做参考，具体纯度与批次有关系，可咨询客服
储存条件 Powder: -20°C for 3 years | In solvent: -80°C for 2 years
别名 (S)-(-)-Blebbistatin

靶点活性

non-muscle myosin II ATPases:0.5 μM-5 μM

体内活性

Blebbistatin completely relaxes both KCl- and carbachol-induced rat detrusor and endothelin-1-induced human bladder contraction in a dose-dependent manner. Pre-incubation with 10 μM blebbistatin attenuates carbachol responsiveness by 65% while blocking electrical field stimulation-induced bladder contraction reaching 50% inhibition at 32 Hz[4].

溶解度

DMSO:29.2 mg/mL (100 mM)

细胞实验

Freshly isolated HSCs are replated on 96-well plate. At day 3, medium is replaced by serum-free medium and cells are starved overnight, treated with or without blebbistatin (25 μM) for 2 h followed by stimulation with platelet-derived growth factor-BB (20 ng/mL). After an overnight incubation, the WST-1 cell proliferation assay are performed[3].

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
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C1   V1   C2   V2