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(-)-Blebbistatin

CAS号

856925-71-8

分子式

C18H16N2O2

主要靶点

Myosin|ATPase

仅限科研使用

Cat No : CM05239

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Synonyms

Inhibitor|Myosin|non-muscle myosin II ATPases|Blebbistatin|(S)-(-)-Blebbistatin|( ) Blebbistatin|()Blebbistatin|inhibit

验证数据展示

  • CAS No.: 856925-71-8

    (-)-Blebbistatin
    CAS#: 856925-71-8

产品信息

CAS号 856925-71-8
分子式 C18H16N2O2
主要靶点 Myosin|ATPase
主要通路 离子通道|细胞骨架
分子量 292.33
纯度 99.59%， 此纯度可做参考，具体纯度与批次有关系，可咨询客服
储存条件 store at low temperature,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
别名 Inhibitor|Myosin|non-muscle myosin II ATPases|Blebbistatin|(S)-(-)-Blebbistatin|( ) Blebbistatin|()Blebbistatin|inhibit

靶点活性

Non-muscle myosin II ATPases:0.5 μM-5 μM

体内活性

方法：为研究对动脉血栓形成的作用，将 (-)-Blebbistatin (1 mg/kg) 腹腔注射给 C57BL/6J 小鼠，30 min 后用 FeCl3 构建颈动脉血栓形成模型。 结果：(-)-Blebbistatin 通过小鼠动脉血管组织中的 GSK3β-NF-κB 信号通路，显著减少 FeCl3 溶液诱导的小鼠颈动脉血栓形成，抑制 NMMHC IIA 的表达并阻碍 TF 的表达。[3]

体外活性

方法：小鼠肝星状细胞 HSCs 用 (-)-Blebbistatin (12.5-50 μM) 处理 2 h，使用 microscopy 观察细胞形态。 结果：当用 (-)-Blebbistatin 处理 2 h，细胞变得更小，获得更多的树突形态，并且具有更少的应力纤维和片状足。[1] 方法：猪 TM 细胞用 (-)-Blebbistatin (25-100 μM) 处理 2 h，使用 Western Blot 检测靶点蛋白表达水平。 结果：对照样品和药物处理样品之间的 MLC 磷酸化状态没有差异。[2]

溶解度

DMSO:55 mg/mL (188.14 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2.92 mg/mL (9.99 mM)

细胞实验

Freshly isolated HSCs are replated on 96-well plate. At day 3, medium is replaced by serum-free medium and cells are starved overnight, treated with or without blebbistatin (25 μM) for 2 h followed by stimulation with platelet-derived growth factor-BB (20 ng/mL). After an overnight incubation, the WST-1 cell proliferation assay are performed[3].

参考文献

1.Liu Z, et al. Blebbistatin inhibits contraction and accelerates migration in mouse hepatic stellate cells. Br J Pharmacol. 2010 Jan 1;159(2):304-15.
2.Zhang M, et al. Blebbistatin, a novel inhibitor of myosin II ATPase activity, increases aqueous humor outflow facility in perfused enucleated porcine eyes. Invest Ophthalmol Vis Sci. 2005 Nov;46(11):4130-8.
3.Zhang Y, et al. The Myosin II Inhibitor, Blebbistatin, Ameliorates FeCl3-induced Arterial Thrombosis via the GSK3β-NF-κB Pathway. Int J Biol Sci. 2017 May 15;13(5):630-639.
4.Zhang X, et al. In vitro and in vivo relaxation of urinary bladder smooth muscle by the selective myosin IIinhibitor, blebbistatin. BJU Int. 2011 Jan;107(2):310-7.
5.Tian X, Gao M, Li A, et al. Protocol for Isolation of Viable Adult Rat Cardiomyocytes with High Yield[J]. STAR Protocols. 2020: 100045.

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
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C1   V1   C2   V2