Recombinant Human BLNK protein (rFc Tag)
种属
Human
纯度
>90 %, SDS-PAGE
标签
rFc Tag
生物活性
未测试
验证数据展示
产品信息
| 纯度 | >90 %, SDS-PAGE |
| 内毒素 | <0.1 EU/μg protein, LAL method |
| 生物活性 |
Not tested |
| 来源 | HEK293-derived Human BLNK protein Ser330-Ser456 (Accession# Q8WV28-1) with a rabbit IgG Fc tag at the C-terminus. |
| 基因ID | 29760 |
| 蛋白编号 | Q8WV28-1 |
| 预测分子量 | 39.9 kDa |
| SDS-PAGE | 42-50 kDa, reducing (R) conditions |
| 组分 | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| 复溶 | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| 储存条件 |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| 运输条件 | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
背景信息
BLNK (also known as SLP-65 or BASH) is an important adaptor protein selectively expressed in B-lineage cells. BLNK bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. BLNK has been shown to be necessary for BCR-mediated Ca2+ mobilization, for the activation of mitogen-activated protein kinases such as ERK, JNK, and p38 in a chicken B cell line DT40, and for activation of transcription factors such as NF-AT and NF-κB in human or mouse B cells.
参考文献:
1. Baba, Y et al. Proc Natl Acad Sci U S A. vol. 98,5 (2001): 2582-6. 2. Nakayama, Joji et al. Blood. 2009;113(7):1483-1492. 3. Minegishi, Y et al.. Science. 1999;286(5446):1954-1957.
