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Recombinant Human ULBP2 protein (His Tag)

种属

Human

纯度

>95 %, SDS-PAGE

标签

His Tag

生物活性

EC50: 18-72 ng/mL

Cat no : Eg0858

Print datasheet

Synonyms

ULBP2, ALCAN alpha, ALCAN-alpha, N2DL2, N2DL-2

验证数据展示

  • Purity of Recombinant Human ULBP2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human ULBP2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human ULBP2(His tag) at 2 μg/mL (100 μL/well) can bind Human NKG2D (hFc tag) with a linear range of 18-72 ng/mL.

    Immobilized Human ULBP2(His tag) at 2 μg/mL (100 μL/well) can bind Human NKG2D (hFc tag) with a linear range of 18-72 ng/mL.

产品信息

纯度 >95 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Immobilized Human ULBP2(His tag) at 2 μg/mL (100 μL/well) can bind Human NKG2D (hFc tag) with a linear range of 18-72 ng/mL.
来源 HEK293-derived Human ULBP2 protein Gly26-Ser217 (Accession# Q9BZM5) with a His tag at the C-terminus.
基因ID 80328
蛋白编号 Q9BZM5
预测分子量 22.5 kDa
SDS-PAGE 26-32 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

NKG2D is an activating cell surface receptor that is predominantly expressed on cytotoxic immune cells. NKG2D recognizes a wide range of ligands. In humans, the NKG2D ligand (NKG2DL) includes MICA, MICB, and six members of the ULBP family (ULBP1-6). ULBPs are MHC class I-related molecules. ULBP2 contains two Ig-like domains, the alpha-1 and alpha-2 domains characteristic of the MHC class I family, but lacks the alpha-3 domain. It can be expressed as either a transmembrane or GPI-linked protein, or released from the cell surface. ULBP2 binds and activates the NKG2D, mediating the recruitment and activation of NK cells.

参考文献:

1.D Cosman, et al. (2001) Immunity. 14(2):123-33.
2.Lola Fernández-Messina, et al. (2011). J Cell Sci. 124(Pt 3):321-7.  
3.Ruipeng Wang, et al. (2014). PLoS One. 9(3):e91133.
4.Felix M Wensveen, et al. (20118) Front Immunol. 8:9:441.
5.Shixin Duan, et al. (2019) Mol Cancer. 18(1):29.