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Recombinant Mouse CD86 protein (His Tag)

种属

Mouse

纯度

>95 %, SDS-PAGE

标签

His Tag

生物活性

EC50: 10-40 ng/mL

Cat no : Eg0802

Print datasheet

Synonyms

B7-2/CD86, Cd86, B7, B7 2, B7.2

验证数据展示

  • Purity of Recombinant Mouse CD86 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse CD86 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Mouse CD86 (His tag) at 1 μg/mL (100 μL/well) can bind Mouse CTLA-4 (hFc tag) with a linear range of 10-40 ng/mL.

    Immobilized Mouse CD86 (His tag) at 1 μg/mL (100 μL/well) can bind Mouse CTLA-4 (hFc tag) with a linear range of 10-40 ng/mL.

产品信息

纯度 >95 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Immobilized Mouse CD86 (His tag) at 1 μg/mL (100 μL/well) can bind Mouse CTLA-4 (hFc tag) with a linear range of 10-40 ng/mL.
来源 HEK293-derived Mouse CD86 protein Val24-Glu245 (Accession# P42082-1) with a His tag at the C-terminus.
基因ID 12524
蛋白编号 P42082-1
预测分子量 26 kDa
SDS-PAGE 32-48 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin (Ig)superfamily. CD86 is primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages.CD86 has strong structural similarity with another B7 family molecule, CD80(B7-1).CD86 and CD80 are the ligands for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte antigen 4 (CTLA-4). Binding of CD86 or  CD80 with CD28 antigen is a costimulatory signal for T cell activation, proliferation, and cytokine production. Binding of CD86 or CD80 with CTLA-4 negatively regulates T cell activation and diminishes the immune response.However, CD86and CD80 bind to CTLA-4 with higher affinity than CD28. Defects in CTLA-4-mediated transendocytosis of CD86 are associated with autoimmunity.

参考文献:

1.Bolandi N, et al. (2021). Int J Mol Sci. 22(19):10719
2.Yokozeki H, et al. (1996). J Invest Dermatol.106(1):147-153
3.Baravalle G, et al. (2011). J Immunol. 187(6):2966-2973. 
4.Collins M, et al. (2005). Genome Biol. 6(6):223
5.Greaves P, et al. (2013). Blood. 121(5):734-744
6.Kennedy A, et al. (2022). Nat Immunol.23(9):1365-1378