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Recombinant Mouse IL-17F protein (His Tag)

种属

Mouse

纯度

>90 %, SDS-PAGE

标签

His Tag

生物活性

EC50: 2-10 ng/mL

Cat no : Eg0671

Print datasheet

Synonyms

IL-17F, Il17f, C87042, IL 17F, interleukin 17F

验证数据展示

  • Purity of Recombinant Mouse IL-17F was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse IL-17F was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Measured by its ability to induce CXCL1 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 2-10 ng/mL.

    Measured by its ability to induce CXCL1 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 2-10 ng/mL.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Measured by its ability to induce CXCL1 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 2-10 ng/mL.
来源 HEK293-derived Mouse IL-17F protein Arg29-Ala161 (Accession# Q7TNI7-1) with His tag at the C-terminus.
基因ID 257630
蛋白编号 Q7TNI7-1
预测分子量 15.7 kDa
SDS-PAGE 18-25 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

The interleukin 17 (IL-17) family of cytokines contains 6 structurally related cytokines, IL-17A, IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. IL-17 family plays crucial roles in host defense against microbial organisms and in the development of inflammatory diseases. IL-17A is a pro-inflammatory cytokine that also has the capacity to promote angiogenesis and osteoclastogenesis. IL-17F shares the highest homology with IL-17A and signals via a receptor composed by the IL-17RA and IL-17RC subunits. IL-17A and IL-17F can form IL-17A/A or IL-17F/F homodimers, IL-17A/F heterodimers are also formed. IL-17A and IL-17F, produced by the Th17 CD4(+) T cell lineage, have been linked to a variety of inflammatory and autoimmune conditions. IL-17F levels are elevated in sera and lesional psoriatic skin compared to non-lesional tissue. IL-17F also has been implicated in the development of neutrophilic airway inflammation.

参考文献:

1. Chunfang Gu, et al. (2013) Cytokine.64(2):477-85.
2. Seon Hee Chang, et al.(2009) Cytokine.46(1):7-11.
3. Askar M Akimzhanov, et al. (2021) J Biol Chem.282(9):5969-72.
4. Catherine Soderstrom, et al. (2017) AAPS J.19(4):1218-1222.
5. Jill F Wright, et al. (2008) J Immunol.181(4):2799-805.