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Recombinant Human IFN-gamma protein (Myc Tag, His Tag)

种属

Human

纯度

>90 %, SDS-PAGE

标签

Myc Tag, His Tag

生物活性

未测试

Cat no : Eg0004

Print datasheet

Synonyms

IFNG, IFG, IFN gamma, IFN γ, IFNγ

验证数据展示

  • Purity of Recombinant Human IFN-gamma was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human IFN-gamma was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 
Not tested
来源 HEK293-derived Human IFN-gamma protein Gln24-Gly161 (Accession# P01579) with a Myc tag and a His tag at the C-terminus.
基因ID 3458
蛋白编号 P01579
预测分子量 21.2 KDa
SDS-PAGE 25-38 kDa, reducing (R) conditions
组分 Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Interferon gamma (IFN γ) is a type II interferon that confers immunity to bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types, including natural killer cells, natural killer T cells and effector lymphocyte T cells, in response to antigenic and inflammatory stimuli. The IFN-γ dimer binds to its cognate receptor, which has two subunits: IFN-γR1, the ligand binding chain (α chain), and IFN-γR2, the signal transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway, which in turn activates IFN-γ responsive genes. While IFN γ can inhibit viral replication, it also acts as an immunomodulator and immunostimulator by increasing the surface expression of class I MHC proteins.

参考文献:

1.Billiau, Alfons et al. Cytokine & growth factor reviews vol. 20,2 (2009): 97-113. 
2.Tau, G et al. Allergy vol. 54,12 (1999): 1233-51.
3.Ding, Huihui et al. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie vol. 155, (2022): 113683.
4.Cordeiro, Patrícia Azevedo Soares et al. Revista do Instituto de Medicina Tropical de Sao Paulo vol. 64, (2022): e64.