验证数据展示
经过测试的应用
| Positive WB detected in | mouse colon tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87446-1-RR targets LYPD8 in WB, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg7518 Product name: Recombinant mouse Lypd8 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 21-255 aa of NM_001083884.1 Sequence: LNCTQCYTYNSTCDGQATECNEQSFSCVESSINSTLGGFLHVYQNKFCSASNCTENSTEVAFTVHLFDDQRYHFASQCCQGESCNATHSESGTQNVTDMQCMSCYGHNKTLCEEKPQKCYEGEQCVFIIAEMVNGSGRVELKGCSDISNSTCQFLSPGNTTVGEFVFKSVECTQPTEYTNSTTTIPPITNTSLTSVTRPGIKTSPASVTPQASMGTKASFTSSIFGSLLLLKLLF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | RIKEN cDNA 2210415F13 gene |
| 别名 | Ly6/PLAUR domain-containing protein 8 |
| 计算分子量 | 28 kDa |
| 观测分子量 | 75 kDa, 45 kDa |
| GenBank蛋白编号 | NM_001083884.1 |
| 基因名称 | 2210415F13Rik |
| Gene ID (NCBI) | 70163 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9D7S0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ly6/PLAUR domain-containing protein 8 (LYPD8) is a highly glycosylated glycosylphosphatidylinositol-anchored protein, which is anchored to intestinal epithelial cells in the uppermost epithelial layer, is constitutively shed into the intestinal lumen and preferentially binds to flagellated bacteria from various genera (PMID: 27027293; 29259722). It has been reported that Lypd8 mediates the segregation of intestinal bacteria and epithelial cells in the colon, thereby preserving intestinal homeostasis (PMID: 27027293). The higher observed molecular weight of LYPD8 is 75 kDa, due to glycosylation modification (PMID: 29259722).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LYPD8 antibody 87446-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
