验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, Caco-2 cells, L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87431-1-RR targets HMBS in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag6509 Product name: Recombinant human HMBS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-361 aa of BC000520 Sequence: MSGNGNAAATAEENSPKMRVIRVGTRKSQLARIQTDSVVATLKASYPGLQFEIIAMSTTGDKILDTALSKIGEKSLFTKELEHALEKNEVDLVVHSLKDLPTVLPPGFTIGAICKRENPHDAVVFHPKFVGKTLETLPEKSVVGTSSLRRAAQLQRKFPHLEFRSIRGNLNTRLRKLDEQQEFSAIILATAGLQRMGWHNRVGQILHPEECMYAVGQGALGVEVRAKDQDILDLVGVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTAMKDGQLYLTGGVWSLDGSDSIQETMQATIHVPAQHEDGPEDDPQLVGITARNIPRGPQLAAQNLGISLANLLLSKGAKNILDVARQLNDAH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | hydroxymethylbilane synthase |
| 别名 | EC:2.5.1.61, hydroxymethylbilane synthase, PBG D, PBGD, PBG-D |
| 计算分子量 | 39 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | BC000520 |
| 基因名称 | HMBS |
| Gene ID (NCBI) | 3145 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P08397 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hydroxymethylbilane synthase (HMBS), also known as porphobilinogen deaminase (PBGD) or previously as uroporphyrinogen I synthase, is a key enzyme in the heme biosynthesis pathway. HMBS is a cytoplasmic enzyme that catalyzes a critical step in the heme biosynthesis pathway: the polymerization of four porphobilinogen molecules to form linear hydroxymethylbilane. It is expressed in all tissues, with the highest levels observed in the liver and erythroid precursors to meet high heme demand. Two main isoforms exist: a ubiquitous "housekeeping" isoform and an erythroid-specific isoform. The observed molecular weight of human HMBS protein is approximately 40-42 kDa (as determined by SDS-PAGE). The erythroid-specific isoform has a slightly lower molecular weight due to a different translation initiation site. (PMID: 19292878, PMID: 12555854)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HMBS antibody 87431-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
