验证数据展示
经过测试的应用
| Positive WB detected in | ACHN cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86987-1-RR targets MUC15 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg5625 Product name: recombinant human MUC15 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 24-236 aa of NM_145650 Sequence: KENQDINTTQNIAEVFKTMENKPISLESEANLNSDKENITTSNLKASHSPPLNLPNNSHGITDFSSNSSAEHSLGSLKPTSTISTSPPLIHSFVSKVPWNAPIADEDLLPISAHPNATPALSSENFTWSLVNDTVKTPDNSSITVSILSSEPTSPSVTPLIVEPSGWLTTNSDSFTGFTPYQEKTTLQPTLKFTNNSKLFPNTSDPQKENRNT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | mucin 15, cell surface associated |
| 别名 | MUC 15, MUC-15, Mucin 15, Mucin-15, PAS3 |
| 计算分子量 | 36 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | NM_145650 |
| 基因名称 | MUC15 |
| Gene ID (NCBI) | 143662 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8N387-1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MUC15 is a transmembrane mucin that is ubiquitously expressed on the surface of epithelial cells as a membrane-bound glycoprotein. MUC15 plays a dual role in cellular processes, participating in cell adhesion, signal transduction, and regulating receptor tyrosine kinase activity. Its expression is frequently altered in various cancers, acting as a context-dependent tumor suppressor or promoter.(PMID: 32479243;16904320; 30420637)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MUC15 antibody 86987-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
