验证数据展示
经过测试的应用
| Positive WB detected in | rat thymus tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86985-1-RR targets CD43 in WB, ELISA applications and shows reactivity with rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | rat |
| 免疫原 |
CatNo: Eg5149 Product name: recombinant rat CD43 protein Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 8-231 aa of P13838 Sequence: ENLPNTMTMLPFTPNSESPSTSEALSTYSSIATVPVTEDPKESISPWGQTTAPASSIPLGTPELSSFFFTSAGASGNTPVPELTTSQEVSTEASLVLFPKSSGVASDPPVTITNPATSSAVASTSLETFKGTSAPPVTVTSSTMTSGPFVATTVSSETSGPPVTMATGSLGPSKETHGLSATIATSSGESSSVAGGTPVFSTKISTTSTPNPITTVPPRPGSSG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | sialophorin |
| 别名 | Spn, gpL115, Lsn, Lsn1, sialophorin |
| 计算分子量 | 38 kDa |
| 观测分子量 | 90-110 kDa |
| GenBank蛋白编号 | P13838 |
| 基因名称 | CD43 |
| Gene ID (NCBI) | 24796 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P13838 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD43, also known as leukosialin, sialophorin, leukocyte sialoglycoprotein, SPN and W3/13 antigen, is a mucin-like type I transmembrane protein. Rat CD43 is a sialoglycoprotein with an apparent molecular weight of ~100 kDa and is found on thymocytes, T lymphocytes, neutrophils, plasma cells and myelomas but not on B lymphocytes or most other tissues (PMID: 2965006). CD43 is involved in the contact between cells participating in a series of events such as signaling, adherence, and host-parasite interactions (PMID: 10580634; 31197200).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD43 antibody 86985-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
