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验证数据展示

  • WB analysis of human plasma using 86500-1-RR

    WB analysis of human plasma using 86500-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86500-1-RR (APOD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of human placenta using 86500-1-RR

    WB analysis of human placenta using 86500-1-RR

    human placenta tissue were subjected to SDS PAGE followed by western blot with 86500-1-RR (APOD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86500-1-RR

    WB analysis using 86500-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86500-1-RR (APOD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 86500-1-RR

    Affinity and Kinetic Characterization of 86500-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86500-1-RR against Human APOD were performed. The affinity constant is below 1 pM.

APOD Recombinant monoclonal antibody

APOD Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 86500-1-RR

产品说明书

CloneNo. 251229A10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Apo D, Apo-D, apolipoprotein D

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman plasma, human placenta tissue, human urine

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86500-1-RR targets APOD in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0812

Product name: Recombinant human APOD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 15-189 aa of BC007402

Sequence: FGAAEGQAFHLGKCPNPPVQENFDVNKYLGRWYEIEKIPTTFENGRCIQANYSLMENGKIKVLNQELRADGTVNQIEGEATPVNLTEPAKLEVKFSWFMPSAPYWILATDYENYALVYSCTCIIQLFHVDFAWILARNPNLPPETVDSLKNILTSNNIDVKKMTVTDQVNCPKLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 apolipoprotein D
别名 Apo D, Apo-D, apolipoprotein D
计算分子量 33 kDa
观测分子量 21-33 kDa
GenBank蛋白编号BC007402
基因名称 APOD
Gene ID (NCBI) 347
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP05090
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Apolipoprotein D (ApoD) is a member of the lipocalin superfamily of ligand transporters, and has been implicated in the transport of small hydrophobic molecules. ApoD is also a component of plasma high-density lipoproteins (HDL). Alteration of ApoD expression has been linked to multiple neurological disorders, including Alzheimer's disease.

实验方案

Product Specific Protocols
WB protocol for APOD antibody 86500-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols