• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 86229-1-RR

    WB analysis using 86229-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86229-1-RR (F11r(CD321) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of bEnd.3 using 86229-1-RR

    IF Staining of bEnd.3 using 86229-1-RR

    Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using JAM-A/CD321 antibody (86229-1-RR, Clone: 250060H5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • WB analysis using 86229-1-RR

    WB analysis using 86229-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86229-1-RR (F11r(CD321) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

JAM-A/CD321 Recombinant monoclonal antibody

JAM-A/CD321 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86229-1-RR

产品说明书

CloneNo. 250060H5

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IF/ICC, ELISA

别名

CD321, F11r, Jam1, JAM-1, JAM-A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inbEnd.3 cells, rat lung tissue, mouse lung tissue, mouse testis tissue, mouse liver tissue
Positive IF/ICC detected inbEnd.3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86229-1-RR targets JAM-A/CD321 in WB, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg3117

Product name: Recombinant Mouse JAM-A/Junctional Adhesion Molecule A protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 27-238 aa of NM_172647.2

Sequence: KGSVYTAQSDVQVPENESIKLTCTYSGFSSPRVEWKFVQGSTTALVCYNSQITAPYADRVTFSSSGITFSSVTRKDNGEYTCMVSEEGGQNYGEVSIHLTVLVPPSKPTISVPSSVTIGNRAVLTCSEHDGSPPSEYSWFKDGISMLTADAKKTRAFMNSSFTIDPKSGDLIFDPVTAFDSGEYYCQAQNGYGTAMRSEAAHMDAVELNVGG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 F11 receptor
别名 CD321, F11r, Jam1, JAM-1, JAM-A
计算分子量32 kDa
观测分子量33 kDa
GenBank蛋白编号NM_172647.2
基因名称 F11r
Gene ID (NCBI) 16456
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO88792
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Junctional Adhesion Molecule-A (JAM-A), also known as CD321 or F11R, is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. It is primarily located in the tight junctions of epithelial and endothelial cells and is also expressed on circulating platelets and leukocytes (PMID: 34533648). Targeting F11R/JAM-A with monoclonal antibodies or peptide antagonists has shown promise in inhibiting tumor growth and metastasis in mouse models (PMID: 37563645).

实验方案

Product Specific Protocols
IF protocol for JAM-A/CD321 antibody 86229-1-RRDownload protocol
WB protocol for JAM-A/CD321 antibody 86229-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...