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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 85389-1-RR

    WB analysis using 85389-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85389-1-RR (Cas9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85389-1-RR

    Affinity and Kinetic Characterization of 85389-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85389-1-RR against Human Cas9 were performed. The affinity constant is 3.18 nM.

Cas9 Recombinant antibody

Cas9 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85389-1-RR

产品说明书

CloneNo. 242781G12

宿主/亚型

Rabbit / IgG

种属反应性

n/a

应用

WB, ELISA

别名

242781G12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85389-1-RR targets Cas9 in WB, ELISA applications and shows reactivity with n/a samples.

经测试应用 WB, ELISA Application Description
经测试反应性 n/a
免疫原 Cas9 fusion protein Ag25281 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Cas9
别名 242781G12
计算分子量 160 kDa
GenBank蛋白编号NP_269215
基因名称
Gene ID (NCBI)
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes. This antibody detects the sp Cas9.

实验方案

Product Specific Protocols
WB protocol for Cas9 antibody 85389-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols