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验证数据展示

  • WB analysis of HeLa using 85256-4-RR

    WB analysis of HeLa using 85256-4-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 85256-4-RR (COL4A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 85256-4-RR

    IHC staining of human colon using 85256-4-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85256-4-RR (COL4A1 antibody) at dilution of 1:1500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 85256-4-RR

    IHC staining of human kidney using 85256-4-RR

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 85256-4-RR (COL4A1 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 85256-4-RR

    IHC staining of human placenta using 85256-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85256-4-RR (COL4A1 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 85256-4-RR

    IHC staining of human placenta using 85256-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85256-4-RR (COL4A1 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85256-4-RR

    Affinity and Kinetic Characterization of 85256-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85256-4-RR against Human COL4A1 were performed. The affinity constant is 1.16 nM.

COL4A1 Recombinant monoclonal antibody

COL4A1 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 85256-4-RR

产品说明书

CloneNo. 251500E12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

arresten, Collagen alpha 1(IV) chain, Collagen alpha-1(IV) chain, Collagen Type IV

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman placenta tissue, human colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:750-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85256-4-RR targets COL4A1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34391

Product name: Recombinant human COL4A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 901-1110 aa of BC151220

Sequence: LPGEKGDHGFPGSSGPRGDPGLKGDKGDVGLPGKPGSMDKVDMGSMKGQKGDQGEKGQIGPIGEKGSRGDPGTPGVPGKDGQAGQPGQPGPKGDPGISGTPGAPGLPGPKGSVGGMGLPGTPGEKGVPGIPGPQGSPGLPGDKGAKGEKGQAGPPGIGIPGLRGEKGDQGIAGFPGSPGEKGEKGSIGIPGMPGSPGLKGSPGSVGYPGS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 collagen, type IV, alpha 1
别名 arresten, Collagen alpha 1(IV) chain, Collagen alpha-1(IV) chain, Collagen Type IV
计算分子量 1669 aa, 161 kDa
观测分子量220 kDa
GenBank蛋白编号BC151220
基因名称 COL4A1
Gene ID (NCBI) 1282
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP02462
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COL4A1 (Collagen Type IV Alpha 1 Chain) encodes a major component of type IV collagen, which is an essential structural element of basement membranes in various tissues. COL4A1 forms heterotrimers with other collagen type IV chains (e.g., COL4A2) and interacts with extracellular matrix components such as laminins, proteoglycans, and perlecans. Proteolytic cleavage of the non-collagenous domain of COL4A1 produces a fragment called arresten, which has anti-angiogenic and tumor suppressor properties. Elevated expression of COL4A1 has been observed in various cancers, including hepatocellular carcinoma (HCC), where it promotes tumor growth and metastasis through the FAK-Src signaling pathway. COL4A1 is highly expressed in various tissues, including the placenta, fat, and other organs. The expression of COL4A1 can be regulated by transcription factors such as RUNX1, which has been shown to upregulate COL4A1 in hepatocellular carcinoma.

实验方案

Product Specific Protocols
IHC protocol for COL4A1 antibody 85256-4-RRDownload protocol
WB protocol for COL4A1 antibody 85256-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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