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验证数据展示

  • WB analysis of MCF-7 using 85189-4-RR

    WB analysis of MCF-7 using 85189-4-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 85189-4-RR (GREB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of T-47D using 85189-4-RR

    WB analysis of T-47D using 85189-4-RR

    T-47D cells were subjected to SDS PAGE followed by western blot with 85189-4-RR (GREB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85189-4-RR

    Affinity and Kinetic Characterization of 85189-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85189-4-RR against Human GREB1 were performed. The affinity constant is 45.5 nM.

GREB1 Recombinant antibody

GREB1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85189-4-RR

产品说明书

CloneNo. 242882D11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

242882D11, GREB1 protein, KIAA0575, Protein GREB1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inT-47D cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85189-4-RR targets GREB1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 GREB1 fusion protein Ag29908 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GREB1 protein
别名 242882D11, GREB1 protein, KIAA0575, Protein GREB1
计算分子量 216 kDa
观测分子量 216-240 kDa
GenBank蛋白编号NM_014668
基因名称 GREB1
Gene ID (NCBI) 9687
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ4ZG55
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GREB1 (Gene regulated by estrogen in breast cancer protein1) encodes a 1949-amino acid protein that contains an N-terminal Zn-binding domain followed by a circularly permuted catalytically inactive superfamily II helicase domain and a C-terminal Fringe-like GT domain. GREB1 is an early estrogen-responsive gene, and its expression is correlated with estrogen levels in breast cancer patients. GREB1 is implicated in hormone-dependent cancers, such as ovarian, prostate cancer and as a risk factor in endometriosis and variations in bone mineral density.

实验方案

Product Specific Protocols
WB protocol for GREB1 antibody 85189-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols