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验证数据展示

  • WB analysis of HeLa using 85148-1-RR (same clone as 85148-1-PBS)

    WB analysis of HeLa using 85148-1-RR (same clone as 85148-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 85148-1-RR (RFC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85148-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85148-1-RR

    Affinity and Kinetic Characterization of 85148-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85148-1-RR against Human RFC1 were performed. The affinity constant is below 1 pM.

RFC1 Recombinant antibody, PBS Only

RFC1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85148-1-PBS

产品说明书

CloneNo. 242818A5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

242818A5, A1, A1 140 kDa subunit, Activator 1 140 kDa subunit, Activator 1 large subunit

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85148-1-PBS targets RFC1 in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原 RFC1 fusion protein Ag13585 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 replication factor C (activator 1) 1, 145kDa
别名 242818A5, A1, A1 140 kDa subunit, Activator 1 140 kDa subunit, Activator 1 large subunit
计算分子量 128 kDa
观测分子量128-150 kDa
GenBank蛋白编号BC051751
基因名称 RFC1
Gene ID (NCBI) 5981
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35251
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Replication factor C subunit 1 (RFC1) is a pentameric DNA polymerase accessory protein involved in DNA replication and repair (PMID: 10051561). It was reported as a cause of cerebellar ataxia with neuropathy and vestibular areflexia syndrome (CANVAS) (PMID: 30926972).