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验证数据展示

  • WB analysis using 85114-1-RR

    WB analysis using 85114-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85114-1-RR (HCLS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat thymus using 85114-1-RR

    WB analysis of rat thymus using 85114-1-RR

    rat thymus tissue were subjected to SDS PAGE followed by western blot with 85114-1-RR (HCLS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse thymus using 85114-1-RR

    IHC staining of mouse thymus using 85114-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 85114-1-RR (HCLS1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse thymus using 85114-1-RR

    IHC staining of mouse thymus using 85114-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 85114-1-RR (HCLS1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse thymus using 85114-1-RR

    WB analysis of mouse thymus using 85114-1-RR

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 85114-1-RR (HCLS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEL using 85114-1-RR

    IF Staining of HEL using 85114-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HEL cells using HCLS1 antibody (85114-1-RR, Clone: 242825E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of Jurkat using 85114-1-RR

    IF Staining of Jurkat using 85114-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using HCLS1 antibody (85114-1-RR, Clone: 242825E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85114-1-RR

    Affinity and Kinetic Characterization of 85114-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85114-1-RR against Human HCLS1 were performed. The affinity constant is 4.71 nM.

HCLS1 Recombinant monoclonal antibody

HCLS1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85114-1-RR

产品说明书

CloneNo. 242825E4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

242825E4, CTTNL, Hematopoietic cell-specific LYN substrate 1, Hematopoietic lineage cell-specific protein, HS1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, mouse thymus tissue, rat thymus tissue, Ramos cells, Daudi cells, Raji cells
Positive IHC detected inmouse thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEL cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85114-1-RR targets HCLS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8645

Product name: Recombinant human HCLS1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 142-486 aa of BC016758

Sequence: GEVEKHTSQKDYSRGFGGRYGVEKDKWDKAALGYDYKGETEKHESQRDYAKGFGGQYGIQKDRVDKSAVGFNEMEAPTTAYKKTTPIEAASSGARGLKAKFESMAEEKRKREEEEKAQQVARRQQERKAVTKRSPEAPQPVIAMEEPAVPAPLPKKISSEAWPPVGTPPSSESEPVRTSREHPVPLLPIRQTLPEDNEEPPALPPRTLEGLQVEEEPVYEAEPEPEPEPEPEPENDYEDVEEMDRHEQEDEPEGDYEEVLEPEDSSFSSALAGSSGCPAGAGAGAVALGISAVALYDYQGEGSDELSFDPDDVITDIEMVDEGWWRGRCHGHFGLFPANYVKLLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hematopoietic cell-specific Lyn substrate 1
别名 242825E4, CTTNL, Hematopoietic cell-specific LYN substrate 1, Hematopoietic lineage cell-specific protein, HS1
计算分子量 486 aa, 54 kDa
观测分子量 75 kDa
GenBank蛋白编号BC016758
基因名称 HCLS1
Gene ID (NCBI) 3059
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP14317
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hematopoietic cell-specific Lyn substrate 1 (HS1, HCLS1, LckBP1 and p75) is a 75-kDa intracellular protein expressed in cells of lymphohemopoietic origin. It was originally identified in B-lymphocytes as a major substrate of B-cell receptor (BCR)-induced phosphorylation after antigen (Ag) stimulation. It's a substrate of the antigen receptor-coupled tyrosine kinase. HCLS1 plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells.

实验方案

Product Specific Protocols
IF protocol for HCLS1 antibody 85114-1-RRDownload protocol
WB protocol for HCLS1 antibody 85114-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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