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验证数据展示

  • WB analysis of Recombinant protein using 84959-4-RR

    WB analysis of Recombinant protein using 84959-4-RR

    Recombinant protein were subjected to SDS PAGE followed by western blot with 84959-4-RR (T7 RNA polymerase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84959-4-RR

    Affinity and Kinetic Characterization of 84959-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84959-4-RR against T7 RNA polymerase were performed. The affinity constant is 1.84 nM.

T7 RNA polymerase Recombinant antibody

T7 RNA polymerase Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84959-4-RR

产品说明书

CloneNo. 242544H3

宿主/亚型

Rabbit / IgG

种属反应性

bacteriophage t7

应用

WB, ELISA

别名

T7 polymerase, 242544H3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRecombinant protein

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84959-4-RR targets T7 RNA polymerase in WB, ELISA applications and shows reactivity with bacteriophage t7 samples.

经测试应用 WB, ELISA Application Description
经测试反应性 bacteriophage t7
免疫原 T7 RNA polymerase fusion protein Ag32001 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 T7 RNA polymerase
别名 T7 polymerase, 242544H3
计算分子量99 kDa
GenBank蛋白编号NP_041960
基因名称 T7 RNA polymerase
Gene ID (NCBI) 1261050
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP00573
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for T7 RNA polymerase antibody 84959-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols