验证数据展示
经过测试的应用
| Positive WB detected in | mouse spleen tissue |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84790-2-RR targets Siglec-E in WB, IHC, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg2733 Product name: Recombinant Mouse Siglec-12 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 19-350 aa of NM_031181.2 Sequence: QNPQEVFTLNVERKVVVQEGLCVLVPCNFSYLKKRLTDWTDSDPVHGFWYREGTDRRKDSIVATNNPIRKAVKETRNRFFLLGDPWRNDCSLNIREIRKKDAGLYFFRLERGKTKYNYMWDKMTLVVTALTNTPQILLPETLEAGHPSNLTCSVPWDCGWTAPPIFSWTGTSVSFLSTNTTGSSVLTITPQPQDHGTNLTCQVTLPGTNVSTRMTIRLNVSYAPKNLTVTIYQGADSVSTILKNGSSLPISEGQSLRLICSTDSYPPANLSWSWDNLTLCPSKLSKPGLLELFPVHLKHGGVYTCQAQHALGSQHISLSLSPQSSATLSEMM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | sialic acid binding Ig-like lectin E |
| 别名 | MIS, mSiglec-E, Myeloid inhibitory siglec, Sialic acid-binding Ig-like lectin 12, Sialic acid-binding Ig-like lectin 5 |
| 计算分子量 | 52 kDa |
| 观测分子量 | 70-90 kDa |
| GenBank蛋白编号 | NM_031181.2 |
| 基因名称 | Siglec-E |
| Gene ID (NCBI) | 83382 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q91Y57 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily that contain sialoside-binding N-terminal domains (PMID: PMID: 9498912; 22749793). Mouse Siglec‑E, also known as myeloid inhibitory siglec (MIS), is a mouse CD33-related siglec that contains three extracellular immunoglobulin-like domains, a transmembrane region and a cytoplasmic tail bearing two immunoreceptor tyrosine-based inhibitory motifs (ITIMs), which can recruit SHP-1 and SHP-2, two inhibitory regulators of immunoreceptor signal transduction (PMID: 11171044). Siglec-E is an inhibitory receptor and is expressed broadly on cells of innate immune cells including neutrophils, monocytes, macrophages, dendritic cells, and a subset of natural killer cells (PMID: 15048729; 31546700).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Siglec-E antibody 84790-2-RR | Download protocol |
| WB protocol for Siglec-E antibody 84790-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
