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验证数据展示

  • WB analysis using 84764-5-RR

    WB analysis using 84764-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84764-5-RR (IARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocellular cancer using 84764-5-RR

    IHC staining of human hepatocellular cancer using 84764-5-RR

    Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 84764-5-RR (IARS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IARS2 antibody included
  • IHC staining of human hepatocellular cancer using 84764-5-RR

    IHC staining of human hepatocellular cancer using 84764-5-RR

    Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer slide using 84764-5-RR (IARS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IARS2 antibody included
  • IF Staining of HepG2 using 84764-5-RR

    IF Staining of HepG2 using 84764-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IARS2 antibody (84764-5-RR, Clone: 242219G4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 84764-5-RR

    IF Staining of HeLa using 84764-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IARS2 antibody (84764-5-RR, Clone: 242219G4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IF Staining of HepG2 using 84764-5-RR

    IF Staining of HepG2 using 84764-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using IARS2 antibody (84764-5-RR, Clone: 242219G4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Affinity and Kinetic Characterization of 84764-5-RR

    Affinity and Kinetic Characterization of 84764-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84764-5-RR against Human IARS2 were performed. The affinity constant is 0.154 nM.

IARS2 Recombinant monoclonal antibody

IARS2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84764-5-RR

产品说明书

CloneNo. 242219G4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

242219G4, EC:6.1.1.5, IleRS, Isoleucine tRNA ligase, Isoleucine--tRNA ligase, mitochondrial

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells
Positive IHC detected inhuman hepatocellular cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy IARS2 Ready-To-Use IHC Kit. IARS2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84764-5-RR targets IARS2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10696

Product name: Recombinant human IARS2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 425-773 aa of BC010218

Sequence: KMSKSLGNVIHPDVVVNGGQDQSKEPPYGADVLRWWVADSNVFTEVAIGPSVLNAARDDISKLRNTLRFLLGNVADFNPETDSIPVNDMYVIDQYMLHLLQDLANKITELYKQYDFGKVVRLLRTFYTRELSNFYFSIIKDRLYCEKENDPKRRSCQTALVEILDVIVRSFAPILPHLAEEVFQHIPYIKEPKSVFRTGWISTSSIWKKPGLEEAVESACAMRDSFLGSIPGKNAAEYKVITVIEPGLLFEIIEMLQSEETSSTSQLNELMMASESTLLAQEPREMTADVIELKGKFLINLEGGDIREESSYKVIVMPTTKEKCPRCWKYTAESSDTLCPRCAEVVSGK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 isoleucyl-tRNA synthetase 2, mitochondrial
别名 242219G4, EC:6.1.1.5, IleRS, Isoleucine tRNA ligase, Isoleucine--tRNA ligase, mitochondrial
计算分子量 1012 aa, 114 kDa
观测分子量114 kDa
GenBank蛋白编号BC010218
基因名称 IARS2
Gene ID (NCBI) 55699
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9NSE4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family.

实验方案

Product Specific Protocols
IF protocol for IARS2 antibody 84764-5-RRDownload protocol
IHC protocol for IARS2 antibody 84764-5-RRDownload protocol
WB protocol for IARS2 antibody 84764-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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