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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84278-5-RR (same clone as 84278-5-PBS)

    WB analysis using 84278-5-RR (same clone as 84278-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84278-5-RR (NME3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84278-5-PBS in a different storage buffer formulation.

  • IHC staining of rat kidney using 84278-5-RR (same clone as 84278-5-PBS)

    IHC staining of rat kidney using 84278-5-RR (same clone as 84278-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 84278-5-RR (NME3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84278-5-PBS in a different storage buffer formulation.

  • IHC staining of rat kidney using 84278-5-RR (same clone as 84278-5-PBS)

    IHC staining of rat kidney using 84278-5-RR (same clone as 84278-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 84278-5-RR (NME3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84278-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84278-5-RR (same clone as 84278-5-PBS)

    IF Staining of HeLa using 84278-5-RR (same clone as 84278-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NME3 antibody (84278-5-RR, Clone: 241585H7 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 84278-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84278-5-RR

    Affinity and Kinetic Characterization of 84278-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84278-5-RR against Human NME3 were performed. The affinity constant is below 1 pM.

NME3 Recombinant antibody, PBS Only

NME3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84278-5-PBS

产品说明书

CloneNo. 241585H7

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

241585H7, DR nm23, DR-nm23, EC:2.7.4.6, KIAA0516

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84278-5-PBS targets NME3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 NME3 fusion protein Ag7284 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 non-metastatic cells 3, protein expressed in
别名 241585H7, DR nm23, DR-nm23, EC:2.7.4.6, KIAA0516
计算分子量 19 kDa
观测分子量 18 kDa
GenBank蛋白编号BC000250
基因名称 NME3
Gene ID (NCBI) 4832
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13232
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NME3 (NME/NM23 Nucleoside Diphosphate Kinase 3), also named as NM23-H3; DR-NM23, is a member of the nucleoside diphosphate kinase (NDPK) family that binds to the mitochondrial outer membrane to stimulate mitochondrial fusion. The depletion of NME3 will cause dysfunction in mitochondrial dynamics. NME3 has been found to facilitate DNA-repair mechanisms binds to several NPHPs, including NEK8, CEP164, and ankyrin repeat and sterile α motif domain-containing 6 (ANKS6)