验证数据展示
经过测试的应用
| Positive IF/ICC detected in | LNCaP cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84016-4-RR targets REPS2 in IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag33764 Product name: Recombinant human REPS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 390-620 aa of NM_004726 Sequence: LPANQQPRDLNRMEKTSVKDMADLPVPNQDVTSDDKQALKSTINEALPKDVSEDPATPKDSNSLKARPRSRSYSSTSIEEAMKRGEDPPTPPPRPQKTHSRASSLDLNKVFQPSVPATKSGLLPPPPALPPRPCPSQSEQVSEAELLPQLSRAPSQAAESSPAKKDVLYSQPPSKPIRRKFRPENQATENQEPSTAASGPASAATMKPHPTVQKQSSKQKKAIQTAIRKNK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | RALBP1 associated Eps domain containing 2 |
| 别名 | REPS 2, RalBP1-interacting protein 2, RalBP1-associated Eps domain-containing protein 2, RalBP1 interacting protein 2, 241136D3 |
| 计算分子量 | 72KD |
| GenBank蛋白编号 | NM_004726 |
| 基因名称 | REPS2 |
| Gene ID (NCBI) | 9185 |
| RRID | AB_3671585 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q8NFH8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
REPS2 is part of a protein complex that regulates the endocytosis of growth factor receptors. The encoded protein directly interacts with a GTPase activating protein that functions downstream of the small G protein Ral. Its expression can negatively affect receptor internalization and inhibit growth factor signaling. There are 4 isoforms of the protein with two short (58 kDa) isoforms comprising 521 or 522 amino acid residues and two long (78 kDa) ones of 659 or 660 residues.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for REPS2 antibody 84016-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
