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验证数据展示

  • IF Staining of HepG2 using 83981-4-RR (same clone as 83981-4-PBS)

    IF Staining of HepG2 using 83981-4-RR (same clone as 83981-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Securin antibody (83981-4-RR, Clone: 241090G10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83981-4-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 83981-4-RR (same clone as 83981-4-PBS)

    FC experiment of Jurkat using 83981-4-RR (same clone as 83981-4-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Securin Recombinant antibody (83981-4-RR, Clone:241090G10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83981-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83981-4-RR

    Affinity and Kinetic Characterization of 83981-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83981-4-RR against Human Securin were performed. The affinity constant is 0.155 nM.

Securin Recombinant antibody, PBS Only

Securin Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83981-4-PBS

产品说明书

CloneNo. 241090G10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

PTTG1, 241090G10, EAP1, Esp1-associated protein, hPTTG

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PTTG1/Securin Ready-To-Use IHC Kit. PTTG1/Securin primary antibody included.

产品信息

83981-4-PBS targets Securin in IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 Securin fusion protein Ag12670 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 pituitary tumor-transforming 1
别名 PTTG1, 241090G10, EAP1, Esp1-associated protein, hPTTG
计算分子量 202 aa, 22 kDa
GenBank蛋白编号BC101834
基因名称 Securin
Gene ID (NCBI) 9232
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95997
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Securin, also known as the pituitary tumor transforming gene (PTTG1), was first isolated from rat pituitary tumor cells. Securin is a multi-functional protein that regulates sister chromatin segregation in mitosis, DNA repair, gene transcription, metabolism, and organ development. Securin is normally expressed in tissues with high proliferative activity, including the spleen, thymus gland, and testis, whereas it is rarely expressed in differentiated mature tissues (PMID: 22563433, PMID: 36123907). The calculated molecular weight of Securin is 22 kDa. With post-translational modification, the molecular weight of Securin will be migrated to 28 kDa.