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验证数据展示

  • IF Staining of HepG2 using 83862-2-RR

    IF Staining of HepG2 using 83862-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FAM127B antibody (83862-2-RR, Clone: 240716B7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HepG2 using 83862-2-RR

    FC experiment of HepG2 using 83862-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug FAM127B Recombinant antibody (83862-2-RR, Clone:240716B7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

FAM127B Recombinant monoclonal antibody

FAM127B Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83862-2-RR

产品说明书

CloneNo. 240716B7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

FAM127, FAM127 A/B/C, 240716B7, CXX1, FAM127A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83862-2-RR targets FAM127B in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag13930

Product name: Recombinant human FAM127B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-113 aa of BC000393

Sequence: MDGRVQLMKALLAGPLRPAARRWRNPIPFPETFDGDTDRLPEFIVQTCSYMFVDENTFSNDALKVTFLITRLTGPALQWVIPYIRKESPLLNDYRGFLAEMKRVFGWEEDEDF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 family with sequence similarity 127, member B
别名 FAM127, FAM127 A/B/C, 240716B7, CXX1, FAM127A
计算分子量 113 aa, 13 kDa
GenBank蛋白编号BC000393
基因名称 FAM127B
Gene ID (NCBI) 26071
RRIDAB_3671447
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9BWD3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The FAM127 family currently has three members, namely FAM127A (also called RTL8C and CXX1), FAM127B (also called CXX1B and RTL8A), and FAM127C (also called RTL8B and CXX1C). They are located on chromosomes and their functions are unknown. All three are composed of 113 amino acids and have extremely high sequence similarity. The immunogen of this antibody has a sequence similarity of more than 95% with members of the FAM127 family.

实验方案

Product Specific Protocols
FC protocol for FAM127B antibody 83862-2-RRDownload protocol
IF protocol for FAM127B antibody 83862-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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