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验证数据展示

  • IF Staining of A431 using 83851-6-RR

    IF Staining of A431 using 83851-6-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PTPN12 antibody (83851-6-RR, Clone: 240983C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of U-251 using 83851-6-RR

    IF Staining of U-251 using 83851-6-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using PTPN12 antibody (83851-6-RR, Clone: 240983C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • WB analysis using 83851-6-RR

    WB analysis using 83851-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83851-6-RR (PTPN12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 83851-6-RR

    IHC staining of human ovary cancer using 83851-6-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83851-6-RR (PTPN12 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83851-6-RR

    Affinity and Kinetic Characterization of 83851-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83851-6-RR against Human PTPN12 were performed. The affinity constant is 48.4 pM.

PTPN12 Recombinant antibody

PTPN12 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83851-6-RR

产品说明书

CloneNo. 240983C9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

PTPG1, PTP PEST, 240983C9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, HEK-293T cells, A549 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, U-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83851-6-RR targets PTPN12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 PTPN12 fusion protein Ag35382 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein tyrosine phosphatase, non-receptor type 12
别名 PTPG1, PTP PEST, 240983C9
计算分子量88kDa
观测分子量110-125 kDa
GenBank蛋白编号NM_002835.3
基因名称 PTPN12
Gene ID (NCBI) 5782
RRIDAB_3671436
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ05209
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN12 (Tyrosine-protein phosphatase non-receptor type 12), also referred to as PTP-PEST (PTP-proline, glutamic acid, serine, and threonine-rich), is a cytoplasmic PTP expressed in a wide spectrum of cell types. PTPN12 has been identified as a potent tumor suppressor in human cancers and a critical regulator of cell adhesion and migration (PMID: 26391955, 29743287). PTPN12 is phosphorylated on serine and threonine residues and is rich in proline (P), glutamate (E), serine (S), and threonine (T) residues. Western blot analysis detected PTPN12 at an apparent molecular mass of 110 kDa.

实验方案

Product Specific Protocols
WB protocol for PTPN12 antibody 83851-6-RRDownload protocol
IHC protocol for PTPN12 antibody 83851-6-RRDownload protocol
IF protocol for PTPN12 antibody 83851-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols