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验证数据展示

  • FC experiment of HEK-293 using 83669-1-RR

    FC experiment of HEK-293 using 83669-1-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Ufd1l Recombinant Antibody (83669-1-RR, Clone:230411A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of HeLa using 83669-1-RR

    FC experiment of HeLa using 83669-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ufd1l Recombinant Antibody (83669-1-RR, Clone:230411A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83669-1-RR

    Affinity and Kinetic Characterization of 83669-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83669-1-RR against Human UFD1L were performed. The affinity constant is below 1 pM.

UFD1L Recombinant antibody

UFD1L Uni-rAbTM Recombinant Antibody for FC (Intra), ELISA
Cat No. 83669-1-RR

产品说明书

CloneNo. 230411A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra), ELISA

别名

UFD1, Ubiquitin recognition factor in ER-associated degradation protein 1, Ubiquitin fusion degradation protein 1, UB fusion protein 1, 230411A2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHEK-293 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy UFD1 Ready-To-Use IHC Kit. UFD1 primary antibody included.

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83669-1-RR targets UFD1L in FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 UFD1L fusion protein Ag0954 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquitin fusion degradation 1 like (yeast)
别名 UFD1, Ubiquitin recognition factor in ER-associated degradation protein 1, Ubiquitin fusion degradation protein 1, UB fusion protein 1, 230411A2
计算分子量 35 kDa
GenBank蛋白编号BC005087
基因名称 UFD1L
Gene ID (NCBI) 7353
RRIDAB_3671274
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ92890
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for UFD1L antibody 83669-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols