验证数据展示
经过测试的应用
| Positive WB detected in | HaCaT cells, HeLa cells, NCCIT cells, MCF-7 cells, Caco-2 cells, HEK-293 cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83666-1-RR targets SLC30A2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | SLC30A2 fusion protein Ag8232 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | solute carrier family 30 (zinc transporter), member 2 |
| 别名 | ZnT-2, ZNT2, ZnT 2, Proton-coupled zinc antiporter SLC30A2, PP12488 |
| 计算分子量 | 323 aa, 35 kDa |
| 观测分子量 | 35 kDa and 52 kDa |
| GenBank蛋白编号 | BC006251 |
| 基因名称 | SLC30A2 |
| Gene ID (NCBI) | 7780 |
| RRID | AB_3671272 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q9BRI3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC30A2, also named as the Zn transporter 2 gene (ZnT2), belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family and SLC30A subfamily. It plays a role in the zinc ion transmembrane transport. Expression of SLC30A2 is restricted to secretory cells, such as acinar pancreatic cells, prostate epithelial cells, placental trophoblasts, Paneth cells, and mammary epithelial cells (MECs). SLC30A2 consists of six transmembrane domains with cytoplasmic N- and C-termini that contain numerous regulatory domains, and function as a homo- or hetero-dimer to transport Zn into vesicles (PMID: 29476070 ). SLC30A2 has 2 isoforms with the molecular mass of 35 kDa (short isoform) and 41 kDa (long isoform). This antibody detects 2 bands of 35 kDa and 52 kDa ( glycosylation form of the long isoform) (PMID: 14608047, PMID: 28174721).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC30A2 antibody 83666-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |