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验证数据展示

  • WB analysis using 83641-4-RR

    WB analysis using 83641-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83641-4-RR (TADA1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of human testis using 83641-4-RR

    WB analysis of human testis using 83641-4-RR

    human testis tissue were subjected to SDS PAGE followed by western blot with 83641-4-RR (TADA1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83641-4-RR

    FC experiment of HepG2 using 83641-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Tada1l Recombinant Antibody (83641-4-RR, Clone:240637F7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • WB analysis of MCF-7 using 83641-4-RR

    WB analysis of MCF-7 using 83641-4-RR

    WB result of TADA1L antibody (83641-4-RR; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-TADA1L transfected MCF-7 cells.

  • IF Staining of MCF-7 using 83641-4-RR

    IF Staining of MCF-7 using 83641-4-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TADA1L antibody (83641-4-RR, Clone: 240637F7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83641-4-RR

    Affinity and Kinetic Characterization of 83641-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83641-4-RR against Human TADA1L were performed. The affinity constant is 0.43 nM.

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  • KD/KO验证

TADA1L Recombinant monoclonal antibody

TADA1L Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83641-4-RR

产品说明书

CloneNo. 240637F7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), ELISA

别名

240637F7, KIAA0764, RP1 9E21.4, SPT3 associated factor 42, SPT3-associated factor 42

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, human testis tissue, HepG2 cells, mouse testis tissue, rat testis tissue
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83641-4-RR targets TADA1L in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag14118

Product name: Recombinant human TADA1L protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-335 aa of BC015401

Sequence: MATFVSELEAAKKNLSEALGDNVKQYWANLKLWFKQKISKEEFDLEAHRLLTQDNVHSHNDFLLAILTRCQILVSTPDGAGSLPWPGGSAAKPGKPKGKKKLSSVRQKFDHRFQPQNPLSGAQQFVAKDPQDDDDLKLCSHTMMLPTRGQLEGRMIVTAYEHGLDNVTEEAVSAVVYAVENHLKDILTSVVSRRKAYRLRDGHFKYAFGSNVTPQPYLKNSVVAYNNLIESPPAFTAPCAGQNPASHPPPDDAEQQAALLLACSGDTLPASLPPVNMYDLFEALQVHREVIPTHTVYALNIERIITKLWHPNHEELQQDKVHRQRLAAKEGLLLC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transcriptional adaptor 1 (HFI1 homolog, yeast)-like
别名 240637F7, KIAA0764, RP1 9E21.4, SPT3 associated factor 42, SPT3-associated factor 42
计算分子量 335 aa, 37 kDa
观测分子量 37-40 kDa
GenBank蛋白编号BC015401
基因名称 TADA1L
Gene ID (NCBI) 117143
RRIDAB_3671252
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96BN2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The function of TADA1L has not been widely studied, and is yet to be fully elucidated. It probably involved in transcriptional regulation.

实验方案

Product Specific Protocols
FC protocol for TADA1L antibody 83641-4-RRDownload protocol
IF protocol for TADA1L antibody 83641-4-RRDownload protocol
WB protocol for TADA1L antibody 83641-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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