验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, HeLa cells, Jurkat cells, HUVEC cells, U-251 cells |
| Positive FC (Intra) detected in | HT-29 cells |
Planning an IHC experiment? We recommend our IHCeasy GBP3 Ready-To-Use IHC Kit. GBP3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
83534-1-RR targets GBP3 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | monkey |
| 免疫原 |
CatNo: Ag33889 Product name: Recombinant human GBP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 535-595 aa of BC140837 Sequence: RERAQLLEEQEKTLTSKLQEQARVLKERCQGESTQLQNEIQKLQKTLKKKTKRYMSHKLKI* 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | guanylate binding protein 3 |
| 别名 | 230178D8, EC:3.6.5.-, GBP 3, GBP-3, GTP binding protein 3 |
| 观测分子量 | 62 kDa, 68 kDa |
| GenBank蛋白编号 | BC140837 |
| 基因名称 | GBP3 |
| Gene ID (NCBI) | 2635 |
| RRID | AB_3671159 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q9H0R5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Guanylate-binding protein 3 (GBP3) is involved in the proliferation of glioma cells through regulating SQSTM1-ERK1/2 pathway. GBP3 has 2 isoforms, one is 595 amino acids long at 68 kDa and the other is at nearly 62 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for GBP3 antibody 83534-1-RR | Download protocol |
| WB protocol for GBP3 antibody 83534-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
