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验证数据展示

  • WB analysis using 83511-1-RR

    WB analysis using 83511-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83511-1-RR (GPR105 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83511-1-RR

    Affinity and Kinetic Characterization of 83511-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83511-1-RR against Human GPR105 were performed. The affinity constant is 3.59 nM.

GPR105 Recombinant antibody

GPR105 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83511-1-RR

产品说明书

CloneNo. 240498B10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

P2RY14, P2Y purinoceptor 14, KIAA0001, G-protein coupled receptor 105, G protein coupled receptor 105

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, MCF-7 cells, human placenta tissue, mouse lung tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83511-1-RR targets GPR105 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 GPR105 fusion protein Ag14105 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 purinergic receptor P2Y, G-protein coupled, 14
别名 P2RY14, P2Y purinoceptor 14, KIAA0001, G-protein coupled receptor 105, G protein coupled receptor 105
计算分子量 338 aa, 39 kDa
观测分子量40 kDa
GenBank蛋白编号BC034989
基因名称 P2RY14
Gene ID (NCBI) 9934
RRIDAB_3671136
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15391
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR105 (P2RY14) is widely expressed throughout many brain regions and peripheral tissues of humans and rodents, and couples to a pertussis toxin-sensitive G protein (PMID: 14559350). GPR105 is also prominently expressed in immune cells, including macrophages, lymphocytes, and neutrophils (PMID: 22778393).

实验方案

Product Specific Protocols
WB protocol for GPR105 antibody 83511-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols