验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, K-562 cells, MCF-7 cells, human placenta tissue, mouse lung tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83511-1-RR targets GPR105 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag14105 Product name: Recombinant human GPR105 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 249-338 aa of BC034989 Sequence: YHIARIPYTKSQTEAHYSCQSKEILRYMKEFTLLLSAANVCLDPIIYFFLCQPFREILCKKLHIPLKAQNDLDISRIKRGNTTLESTDTL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | purinergic receptor P2Y, G-protein coupled, 14 |
| 别名 | P2RY14, P2Y purinoceptor 14, KIAA0001, G-protein coupled receptor 105, G protein coupled receptor 105 |
| 计算分子量 | 338 aa, 39 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC034989 |
| 基因名称 | P2RY14 |
| Gene ID (NCBI) | 9934 |
| RRID | AB_3671136 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q15391 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPR105 (P2RY14) is widely expressed throughout many brain regions and peripheral tissues of humans and rodents, and couples to a pertussis toxin-sensitive G protein (PMID: 14559350). GPR105 is also prominently expressed in immune cells, including macrophages, lymphocytes, and neutrophils (PMID: 22778393).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GPR105 antibody 83511-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
