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验证数据展示

  • WB analysis using 83510-1-RR

    WB analysis using 83510-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83510-1-RR (MED21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 83510-1-RR

    WB analysis of HepG2 using 83510-1-RR

    WB result of MED21 antibody (83510-1-RR; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-MED21 transfected HepG2 cells.

  • FC experiment of HeLa using 83510-1-RR

    FC experiment of HeLa using 83510-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MED21 Recombinant antibody (83510-1-RR, Clone:240419E2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83510-1-RR

    Affinity and Kinetic Characterization of 83510-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83510-1-RR against Human MED21 were performed. The affinity constant is 2.06 nM.

  • Featured Product
  • KD/KO验证

MED21 Recombinant antibody

MED21 Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83510-1-RR

产品说明书

CloneNo. 240419E2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

SURB7, SRB7, hSrb7, 240419E2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, L02 cells, HEK-293T cells
Positive FC (Intra) detected inHeLa cells, U-87 MG cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83510-1-RR targets MED21 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 MED21 fusion protein Ag8568 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mediator complex subunit 21
别名 SURB7, SRB7, hSrb7, 240419E2
计算分子量 144 aa, 16 kDa
观测分子量13-16 kDa
GenBank蛋白编号BC008380
基因名称 MED21
Gene ID (NCBI) 9412
RRIDAB_3671135
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13503
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MED21, also known as SRB7 and SURB7,belongs to the Mediator complex subunit 21 family. MED21 is a small essential subunit of the middle domain of the Mediator, which is conserved in all eukaryotes. MED21 is thought to play an important role in transcriptional activation and repression. MED21 regulates the transcription of nearly all RNA polymerase II-dependent genes (PMID: 16758199, PMID: 30559736).

实验方案

Product Specific Protocols
WB protocol for MED21 antibody 83510-1-RRDownload protocol
FC protocol for MED21 antibody 83510-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols