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验证数据展示

  • WB analysis using 83509-5-RR

    WB analysis using 83509-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83509-5-RR (FBXO38 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 83509-5-RR

    IF Staining of HepG2 using 83509-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FBXO38 antibody (83509-5-RR, Clone: 240513A4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83509-5-RR

    Affinity and Kinetic Characterization of 83509-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83509-5-RR against Human FBXO38 were performed. The affinity constant is 0.233 nM.

FBXO38 Recombinant monoclonal antibody

FBXO38 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83509-5-RR

产品说明书

CloneNo. 240513A4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

SP329, MOKA, Fbx38, F box protein 38, F box only protein 38

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83509-5-RR targets FBXO38 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35444

Product name: Recombinant human FBXO38 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 490-700 aa of XM_005268513.1

Sequence: SNQNSNNDDNNAQNNNANIHDNNHHHPDDSDEENDFRQDLQPGEQQFAADALNEMEDIVQEDGEVVAESGNNTPAHSQAIIPVDVDEEQAGPSGLQRVVKPTSITVHDSESDDEEDSLELQEVWIPKNGTRRYSEREEKTGESVQSRELSVSGKGKTPLRKRYNSHQMGQSKQFPLEESSCEKGCQVTSEQIKADMKAARDIPEKKKNKDV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 F-box protein 38
别名 SP329, MOKA, Fbx38, F box protein 38, F box only protein 38
计算分子量134kDa，1188aa
观测分子量125kDa, 140 kDa, 150kDa
GenBank蛋白编号XM_005268513.1
基因名称 FBXO38
Gene ID (NCBI) 81545
RRIDAB_3671133
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ6PIJ6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

F-box-containing protein 38 (FBXO38), a member of the F-box family, was initially discovered as a co-activator of the transcription factor Krüppel-like factor 7 (KLF7) (PMID: 14729953). FBXO38 acts as a substrate-binding receptor for multi-subunit ubiquitin ligase SKP1-CUL1-FBXO38 (PMID: 35769260) . FBXO38 is an E3 ligase of PD-1 that mediates Lys48-linked poly-ubiquitination and subsequent proteasome degradation (PMID: 30487606).

实验方案

Product Specific Protocols
IF protocol for FBXO38 antibody 83509-5-RRDownload protocol
WB protocol for FBXO38 antibody 83509-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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