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验证数据展示

  • WB analysis using 83443-6-RR

    WB analysis using 83443-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83443-6-RR (NEDD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 83443-6-RR

    IF Staining of HeLa using 83443-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NEDD1 antibody (83443-6-RR, Clone: 240404A7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HEK-293 using 83443-6-RR

    FC experiment of HEK-293 using 83443-6-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Nedd1 Recombinant Antibody (83443-6-RR, Clone:240404A7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of A431 using 83443-6-RR

    FC experiment of A431 using 83443-6-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Nedd1 Recombinant Antibody (83443-6-RR, Clone:240404A7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83443-6-RR

    Affinity and Kinetic Characterization of 83443-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83443-6-RR against Human NEDD1 were performed. The affinity constant is below 1 pM.

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NEDD1 Recombinant monoclonal antibody

NEDD1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83443-6-RR

产品说明书

CloneNo. 240404A7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

240404A7, NEDD 1, NEDD-1, Neural precursor cell expressed developmentally down-regulated protein 1, Protein NEDD1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSH-SY5Y cells, HEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA431 cells, HEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy NEDD1 Ready-To-Use IHC Kit. NEDD1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83443-6-RR targets NEDD1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag33603

Product name: Recombinant human NEDD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-332 aa of BC027605

Sequence: MQENLRFASSGDDIKIWDASSMTLVDKFNPHTSPHGISSICWSSNNNFLVTASSSGDKIVVSSCKCKPVPLLELAEGQKQTCVNLNSTSMYLVSGGLNNTVNIWDLKSKRVHRSLKDHKDQVTCVTYNWNDCYIASGSLSGEIILHSVTTNLSSTPFGHGSNQSVRHLKYSLFKKSLLGSVSDNGIVTLWDVNSQSPYHNFDSVHKAPASGICFSPVNELLFVTIGLDKRIILYDTSSKKLVKTLVADTPLTAVDFMPDGATLAIGSSRGKIYQYDLRMLKSPVKTISAHKTSVQCIAFQYSTVLTKSSLNKGCSNKPTTVNKRSVNVNAAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 neural precursor cell expressed, developmentally down-regulated 1
别名 240404A7, NEDD 1, NEDD-1, Neural precursor cell expressed developmentally down-regulated protein 1, Protein NEDD1
计算分子量 72 kDa
观测分子量71-75 kDa
GenBank蛋白编号BC027605
基因名称 NEDD1
Gene ID (NCBI) 121441
RRIDAB_3671084
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ8NHV4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEDD1 (NEDD1 gamma-tubulin ring complex targeting factor), also known as GCP-WD. It is expected to be located in centrosome; nucleoplasm; and plasma membrane. Required for mitosis progression. Promotes the nucleation of microtubules from the spindle. The molecular weight of NEDD1is 72 kDa.

实验方案

Product Specific Protocols
WB protocol for NEDD1 antibody 83443-6-RRDownload protocol
IF protocol for NEDD1 antibody 83443-6-RRDownload protocol
FC protocol for NEDD1 antibody 83443-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols