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验证数据展示

  • IF Staining of mouse eye using 83364-4-RR

    IF Staining of mouse eye using 83364-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PCARE antibody (83364-4-RR, Clone: 240352F6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse eye using 83364-4-RR

    IF Staining of mouse eye using 83364-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PCARE antibody (83364-4-RR, Clone: 240352F6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 83364-4-RR

    WB analysis using 83364-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83364-4-RR (PCARE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse eye using 83364-4-RR

    IHC staining of mouse eye using 83364-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 83364-4-RR (PCARE antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse eye using 83364-4-RR

    IHC staining of mouse eye using 83364-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 83364-4-RR (PCARE antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83364-4-RR

    Affinity and Kinetic Characterization of 83364-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83364-4-RR against Human PCARE were performed. The affinity constant is 3.06 nM.

PCARE Recombinant monoclonal antibody

PCARE Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83364-4-RR

产品说明书

CloneNo. 240352F6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

C2orf71, 240352F6

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse retina tissue, mouse eye tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83364-4-RR targets PCARE in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag34774

Product name: Recombinant human PCARE protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 10-150 aa of NM_001029883

Sequence: LVNSVAKSGIQFLKKPKAIRPGCQGGSERGSIPLLVKNSTCYDAGEGLAEEQPSPRRNQTTAKGLCQLMGDPASGKRKDMEGLIPGTKTSSSQLNKSQSHMAKDIPFKTQGSHGSQGADFSGDESEESSTQDTSKWKRTAK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromosome 2 open reading frame 71
别名 C2orf71, 240352F6
计算分子量140 kDa
观测分子量140 kDa
GenBank蛋白编号NM_001029883
基因名称 PCARE
Gene ID (NCBI) 388939
RRIDAB_3671018
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDA6NGG8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C2orf71/PCARE (photoreceptor cilium actin regulator) is a retina-specific protein consisting of 1289 amino acids with a predicted molecular weight of 140 kDa. PCARE is predicted to be myristoylated and palmitoylated at its N terminus and plays an important role in delivering actin-associated components to the base of the photoreceptor OSs to regulate the initial development of OS disks (PMID: 32312818, 35253837).

实验方案

Product Specific Protocols
IF protocol for PCARE antibody 83364-4-RRDownload protocol
IHC protocol for PCARE antibody 83364-4-RRDownload protocol
WB protocol for PCARE antibody 83364-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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