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验证数据展示

  • IHC staining of human colon cancer using 83330-2-RR (same clone as 83330-2-PBS)

    IHC staining of human colon cancer using 83330-2-RR (same clone as 83330-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83330-2-RR (Bcl-XL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83330-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCL2L1 antibody included
  • IF Staining of HeLa using 83330-2-RR (same clone as 83330-2-PBS)

    IF Staining of HeLa using 83330-2-RR (same clone as 83330-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Bcl-xL antibody (83330-2-RR, Clone: 240450A9 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83330-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83330-2-RR (same clone as 83330-2-PBS)

    FC experiment of HeLa using 83330-2-RR (same clone as 83330-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Bcl-xL Recombinant antibody (83330-2-RR, Clone:240450A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83330-2-PBS in a different storage buffer formulation.

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BCL2L1 Recombinant monoclonal antibody, PBS Only

BCL2L1 Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83330-2-PBS

产品说明书

CloneNo. 240450A9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

Bcl-xL, 240450A9, Apoptosis regulator Bcl X, Apoptosis regulator Bcl-X, Bcl2-L-1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy BCL2L1 Ready-To-Use IHC Kit. BCL2L1 primary antibody included.

产品信息

83330-2-PBS targets BCL2L1 in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag25604

Product name: Recombinant human Bcl-xL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-128 aa of BC019307

Sequence: MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 BCL2-like 1
别名 Bcl-xL, 240450A9, Apoptosis regulator Bcl X, Apoptosis regulator Bcl-X, Bcl2-L-1
计算分子量 26 kDa
GenBank蛋白编号BC019307
基因名称 BCL2L1
Gene ID (NCBI) 598
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ07817
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L), Bcl-X(s), and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-X(L) isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can form heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-X(s) isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. Bcl-X(beta) is a 227 amino acid protein.