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验证数据展示

  • WB analysis using 83324-1-RR

    WB analysis using 83324-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83324-1-RR (GANAB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 83324-1-RR

    IHC staining of mouse liver using 83324-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83324-1-RR (GANAB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GANAB antibody included
  • FC experiment of HeLa using 83324-1-RR

    FC experiment of HeLa using 83324-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug GANAB Recombinant antibody (83324-1-RR, Clone:240279B7)(red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of HeLa using 83324-1-RR

    WB analysis of HeLa using 83324-1-RR

    WB result of GANAB antibody (83324-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GANAB transfected HeLa cells.

  • IF Staining of HeLa using 83324-1-RR

    IF Staining of HeLa using 83324-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GANAB antibody (83324-1-RR, Clone: 240279B7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83324-1-RR

    Affinity and Kinetic Characterization of 83324-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83324-1-RR against Human GANAB were performed. The affinity constant is 0.186 nM.

  • Featured Product
  • KD/KO验证

GANAB Recombinant antibody

GANAB Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83324-1-RR

产品说明书

CloneNo. 240279B7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

Glucosidase II subunit alpha, G2AN, EC:3.2.1.207, Alpha-glucosidase 2, Alpha glucosidase 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A431 cells, HepG2 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue, human placenta tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GANAB Ready-To-Use IHC Kit. GANAB primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83324-1-RR targets GANAB in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GANAB fusion protein Ag30417 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glucosidase, alpha; neutral AB
别名 Glucosidase II subunit alpha, G2AN, EC:3.2.1.207, Alpha-glucosidase 2, Alpha glucosidase 2
计算分子量944aa,107 kDa; 488aa,55 kDa
观测分子量110 kDa
GenBank蛋白编号BC017435
基因名称 GANAB
Gene ID (NCBI) 23193
RRIDAB_3670990
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14697
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GANAB antibody 83324-1-RRDownload protocol
IHC protocol for GANAB antibody 83324-1-RRDownload protocol
IF protocol for GANAB antibody 83324-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols