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验证数据展示

  • FC experiment of HepG2 using 83217-5-RR

    FC experiment of HepG2 using 83217-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NUDCD2 Recombinant antibody (83217-5-RR, Clone:230513A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • WB analysis of HEK-293 using 83217-5-RR

    WB analysis of HEK-293 using 83217-5-RR

    WB result of NUDCD2 antibody (83217-5-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUDCD2 transfected HEK-293 cells.

  • WB analysis using 83217-5-RR

    WB analysis using 83217-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83217-5-RR (NUDCD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 83217-5-RR

    IP experiment of HepG2 using 83217-5-RR

    IP result of anti-NUDCD2 (IP:83217-5-RR, 4ug; Detection:83217-5-RR 1:4000) with HepG2 cells lysate 1085 ug.

  • IF Staining of HepG2 using 83217-5-RR

    IF Staining of HepG2 using 83217-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUDCD2 antibody (83217-5-RR, Clone: 230513A5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83217-5-RR

    Affinity and Kinetic Characterization of 83217-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83217-5-RR against Human NUDCD2 were performed. The affinity constant is below 1 pM.

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NUDCD2 Recombinant monoclonal antibody

NUDCD2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Cat No. 83217-5-RR

产品说明书

CloneNo. 230513A5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), IP, ELISA

别名

230513A5, NudC domain containing 2, NudC domain-containing protein 2, NudCL2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, K-562 cells, HepG2 cells, U-251 cells
Positive IP detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83217-5-RR targets NUDCD2 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag15579

Product name: Recombinant human NUDCD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-157 aa of BC017934

Sequence: MSAPFEERSGVVPCGTPWGQWYQTLEEVFIEVQVPPGTRAQDIQCGLQSRHVALSVGGREILKGKLFDSTIADEGTWTLEDRKMVRIVLTKTKRDAANCWTSLLESEYAADPWVQDQMQRKLTLERFQKENPGFDFSGAEISGNYTKGGPDFSNLEK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NudC domain containing 2
别名 230513A5, NudC domain containing 2, NudC domain-containing protein 2, NudCL2
计算分子量 157 aa, 18 kDa
观测分子量 18 kDa
GenBank蛋白编号BC017934
基因名称 NUDCD2
Gene ID (NCBI) 134492
RRIDAB_3670903
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ8WVJ2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NUDCD2, also known as NudCL2, is an HSP90 cochaperone that regulates sister chromatid cohesion during mitosis. NUDCD2 also plays a role in ciliogenesis(PMID: 30368549, 34480124). NUDCD2 regulates the LIS1/dynein pathway by stabilizing LIS1 with Hsp90 chaperone(PMID: 20133715).

实验方案

Product Specific Protocols
IF protocol for NUDCD2 antibody 83217-5-RRDownload protocol
IP protocol for NUDCD2 antibody 83217-5-RRDownload protocol
WB protocol for NUDCD2 antibody 83217-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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