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验证数据展示

  • WB analysis using 83121-4-RR

    WB analysis using 83121-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83121-4-RR (GCNT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83121-4-RR

    Affinity and Kinetic Characterization of 83121-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83121-4-RR against Human GCNT1 were performed. The affinity constant is below 1 pM.

GCNT1 Recombinant antibody

GCNT1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83121-4-RR

产品说明书

CloneNo. 240071E9

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

Core 2 beta-1,6-N-acetylglucosaminyltransferase, C2GNT-L, C2GNT1, C2GlcNAcT, Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83121-4-RR targets GCNT1 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 GCNT1 fusion protein Ag12264 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glucosaminyl (N-acetyl) transferase 1, core 2 (beta-1,6-N-acetylglucosaminyltransferase)
别名 Core 2 beta-1,6-N-acetylglucosaminyltransferase, C2GNT-L, C2GNT1, C2GlcNAcT, Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
计算分子量 428 aa, 50 kDa
观测分子量50 kDa
GenBank蛋白编号BC074885
基因名称 GCNT1
Gene ID (NCBI) 2650
RRIDAB_3670831
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ02742
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glucosaminyl (N-acetyl) transferase 1 (GCNT1, also known as C2GNT1), is a glycosyltransferase enzyme that plays an essential role in the formation of core 2 branched O-glycans that is crucial to the final definition of O-glycan structure (PMID: 37813880). GCNT1 promotes the growth of prostate tumors and regulates oncogenic gene expression pathways that are important for disease progression (PMID: 24854630).

实验方案

Product Specific Protocols
WB protocol for GCNT1 antibody 83121-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols