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验证数据展示

  • FC experiment of PC-3 using 82924-1-RR

    FC experiment of PC-3 using 82924-1-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug NFX1 Recombinant antibody (82924-1-RR, Clone:230211D9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • IF Staining of HeLa using 82924-1-RR

    IF Staining of HeLa using 82924-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NFX1 antibody (82924-1-RR, Clone: 230211D9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

NFX1 Recombinant monoclonal antibody

NFX1 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 82924-1-RR

产品说明书

CloneNo. 230211D9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

EC:2.3.2.-, NFX2, Nuclear transcription factor, X box-binding protein 1, Transcriptional repressor NF-X1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82924-1-RR targets NFX1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag9448

Product name: Recombinant human NFX1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 771-1120 aa of BC012151

Sequence: TCARVHECDHPVYHSCHSEEKCPPCTFLTQKWCMGKHEFRSNIPCHLVDISCGLPCSATLPCGMHKCQRLCHKGECLVDEPCKQPCTTPRADCGHPCMAPCHTSSPCPVTACKAKVELQCECGRRKEMVICSEASSTYQRIAAISMASKITDMQLGGSVEISKLITKKEVHQARLECDEECSALERKKRLAEAFHISEDSDPFNIRSSGSKFSDSLKEDARKDLKFVSDVEKEMETLVEAVNKGKNSKKSHSFPPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAIRGKSVCPPTTLTGVLEREMQARPPPPIPHHRHQSDKNPGSSNLQKITKEPIIDYFDVQD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 nuclear transcription factor, X-box binding 1
别名 EC:2.3.2.-, NFX2, Nuclear transcription factor, X box-binding protein 1, Transcriptional repressor NF-X1
计算分子量 1120 aa, 124 kDa
GenBank蛋白编号BC012151
基因名称 NFX1
Gene ID (NCBI) 4799
RRIDAB_3670659
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ12986
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for NFX1 antibody 82924-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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