验证数据展示
经过测试的应用
| Positive IHC detected in | mouse pituitary gland tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse pituitary gland tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82498-2-RR targets ACTH in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, IF-P, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | proopiomelanocortin |
| 别名 | POMC, ACTH Melanotropin alpha, Adrenocorticotropic hormone, Beta LPH Lipotropin gamma, Beta-endorphin |
| 计算分子量 | 267 aa, 29 kDa |
| GenBank蛋白编号 | BC065832 |
| 基因名称 | POMC |
| Gene ID (NCBI) | 5443 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P01189 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Pro-opiomelanocortin (POMC) is a prohormone that encodes multiple smaller peptide hormones within its structure. POMC plays important roles in many pathological and physiological processes, including regulating energy metabolism, immune reactions, and sexual functions. POMC expression differs quantitatively and qualitatively among most normal tissues. The large molecule of POMC is the source of several important biologically active substances. POMC can be cleaved enzymatically into the following peptides: d NPP, γ-MSH, ACTH, α-MSH, CLIP, Lipotropin β, Lipotropin γ, β-MSH, β endorphin and Met-enkephalin.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ACTH antibody 82498-2-RR | Download protocol |
| IHC protocol for ACTH antibody 82498-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
