验证数据展示
经过测试的应用
| Positive WB detected in | H2O2 treated Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
81767-2-RR targets Phospho-ZAP70 (Tyr319)/SYK (Tyr352) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | zeta-chain (TCR) associated protein kinase 70kDa |
| 别名 | Phospho Syk (Tyr352), Phospho ZAP70 (Tyr319)/Syk (Tyr352), Phospho Zap-70 (Tyr319)/Syk (Tyr352), Phospho-Syk (Tyr352), Phospho-Syk-Tyr352 |
| 计算分子量 | 70 kDa |
| 观测分子量 | 70 kDa, 72kDa |
| GenBank蛋白编号 | BC039039 |
| 基因名称 | ZAP70 |
| Gene ID (NCBI) | 7535 |
| ENSEMBL Gene ID | ENSG00000115085 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P43403 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. Three tyrosine residues (Tyr292, Tyr315 and Tyr319) in the interdomain B region of ZAP‐70 have attracted particular attention as sites that might regulate ZAP‐70 activity and/or binding to heterologous proteins. The third candidate phosphorylation site in the interdomain B region of ZAP‐70 is Tyr319. Although the functional importance of this site in ZAP‐70 is unknown, a previous report presented indirect evidence that the orthologous Tyr352 residue in Syk is involved in the association of PLC‐γ1 with this PTK. (PMID: 10202147)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-ZAP70 (Tyr319)/SYK (Tyr352) antibody 81767-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
