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验证数据展示

  • WB analysis using 68877-1-Ig

    WB analysis using 68877-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68877-1-Ig (ADCK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

ADCK4 Monoclonal antibody

ADCK4 Monoclonal Antibody for WB, ELISA
Cat No. 68877-1-Ig

产品说明书

CloneNo. 1B7E3

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, ELISA

别名

EC:2.7.-.-, COQ8B, Coenzyme Q protein 8B, Atypical kinase COQ8B, mitochondrial, AarF domain-containing protein kinase 4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68877-1-Ig targets ADCK4 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 ADCK4 fusion protein Ag13188 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 aarF domain containing kinase 4
别名 EC:2.7.-.-, COQ8B, Coenzyme Q protein 8B, Atypical kinase COQ8B, mitochondrial, AarF domain-containing protein kinase 4
计算分子量 544 aa, 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC013114
基因名称 ADCK4
Gene ID (NCBI) 79934
RRIDAB_3670444
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ96D53
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADCK4 (AarF domain-containing protein kinase 4), also known as COQ8B (Coenzyme Q protein 8B), is an atypical kinase involved in the biosynthesis of coenzyme Q, which is also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration (PMID: 24270420). Its substrate specificity is unclear: does not show any protein kinase activity. It probably acts as a small molecule kinase, possibly a lipid kinase that phosphorylates a prenyl lipid in the ubiquinone biosynthesis pathway (PMID: 24270420).

实验方案

Product Specific Protocols
WB protocol for ADCK4 antibody 68877-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols