验证数据展示
经过测试的应用
| Positive WB detected in | Calu-3 cells, A549 cells, Caco-2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68711-1-Ig targets ACY3 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag10234 Product name: Recombinant human ACY3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-319 aa of BC008689 Sequence: MCSLPVPREPLRRVAVTGGTHGNEMSGVYLARHWLHAPAELQRASFSAVPVLANPAATSGCRRYVDHDLNRTFTSSFLNSRPTPDDPYEVTRARELNQLLGPKASGQAFDFVLDLHNTTANMGTCLIAKSSHEVFAMHLCRHLQLQYPELSCQVFLYQRSGEESYNLDSVAKNGLGLELGPQPQGVLRADIFSRMRTLVATVLDFIELFNQGTAFPAFEMEAYRPVGVVDFPRTEAGHLAGTVHPQLQDRDFQPLQPGAPIFQMFSGEDLLYEGESTVYPVFINEAAYYEKGVAFVQTEKFTFTVPAMPALTPAPSPAS 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | aspartoacylase (aminocyclase) 3 |
| 别名 | ACY 3, ACY3, Acylase III, Aminoacylase 3, ASPA2, Aspartoacylase 2, HCBP1 |
| 计算分子量 | 319 aa, 35 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC008689 |
| 基因名称 | ACY3 |
| Gene ID (NCBI) | 91703 |
| RRID | AB_3670404 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q96HD9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACY3 is a 319 amino acid protein that deacetylates mercapturic acids in the proximal tubules of the kidney, where it is predominantly expressed.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ACY3 antibody 68711-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
