验证数据展示
经过测试的应用
| Positive WB detected in | HT-1376 cells, SW 1990 cells, Saos-2 cells, HEK-293 cells, Caco-2 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
68550-1-Ig targets MSX2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, rat |
| 免疫原 |
CatNo: Ag33220 Product name: Recombinant human MSX2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-267 aa of BC015509 Sequence: MASPSKGNDLFSPDEEGPAVVAGPGPGPGGAEGAAEERRVKVSSLPFSVEALMSDKKPPKEASPLPAESASAGATLRPLLLSGHGAREAHSPGPLVKPFETASVKSENSEDGAAWMQEPGRYSPPPRHTSPTTCTLRKHKTNRKPRTPFTTSQLLALERKFRQKQYLSIAERAEFSSSLNLTETQVKIWFQNRRAKAKRLQEAELEKLKMAAKPMLPSSFSLPFPISSPLQAASIYGASYPFHRPVLPIPPVGLYATPVGYGMYHLS 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | msh homeobox 2 |
| 别名 | 1B1G3, Homeobox protein Hox-8, Homeobox protein MSX-2, HOX8 |
| 计算分子量 | 29 kDa |
| 观测分子量 | 28-35 kDa |
| GenBank蛋白编号 | BC015509 |
| 基因名称 | MSX2 |
| Gene ID (NCBI) | 4488 |
| RRID | AB_3085254 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P35548 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MSX2 (Msh Homeobox 2) is a transcription factor belonging to the MSX homeobox gene family, which plays crucial roles in embryonic development, cell differentiation, and tissue homeostasis. The apparent molecular weight is larger than the calculated molecular weight of 29 kDa, which is likely due to posttranslational modification.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MSX2 antibody 68550-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
