验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, MDA-MB-231 cells, Jurkat cells, MOLT-4 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 clls |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68490-1-Ig targets ABCF2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 |
CatNo: Ag33824 Product name: Recombinant human ABCF2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 2-216 aa of BC001661 Sequence: PSDLAKKKAAKKKEAAKARQRPRKGHEENGDVVTEPQVAEKNEANGRETTEVDLLTKELEDFEMKKAAARAVTGVLASHPNSTDVHIINLSLTFHGQELLSDTKLELNSGRRYGLIGLNGIGKSMLLSAIGKREVPIPEHIDIYHLTREMPPSDKTPLHCVMEVDTERAMLEKEAERLAHEDAECEKLMELYERLEELDADKAEMRASRILHGLG 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ATP-binding cassette, sub-family F (GCN20), member 2 |
| 别名 | ABC28, ABCF2, DKFZp586K1823, EST133090, HUSSY 18, HUSSY18, M ABC1 |
| 计算分子量 | 71 kDa |
| 观测分子量 | 71 kDa |
| GenBank蛋白编号 | BC001661 |
| 基因名称 | ABCF2 |
| Gene ID (NCBI) | 10061 |
| RRID | AB_3085201 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UG63 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ABCF2 (ABC28, M-ABC1, HUSSY-18, EST133090, DKFZp586K1823) is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins are composed of transmembrane domains (TMDs), and nucleotide binding domains (NBDs, or ATP-binding cassettes, ABSs). Based on their sequence similarity scores, the members of the human ABC protein family can be grouped into eight subfamilies, including the MDR/TAP, the ALD, the MRP/CFTR, the ABC1, the White, the RNAseL inhibitor, the ANSA, and the GCN20 subfamilies. ABCF2 is a member of the GCN20 subfamily. ABC proteins transport various molecules across extra- and intracellular membranes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ABCF2 antibody 68490-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
